2016年5月18日 · Fuchou Tang and colleagues have isolated the earliest haematopoietic stem cell (HSC) precursors — rare CD45-negative pre-HSCs — from developing mouse embryos using a combination of surface ...

2024年3月19日 · 该研究深入认识了Pre-HSC成熟的精准调控机制，为HSC体外再生的研究提供了重要的理论依据。 首先，作者通过LC3-RFP-EGFP的小鼠模型，揭示小鼠胚胎AGM区EHT过程相关细胞群：内皮细胞和Pre-HSC的自噬状态存在差异。

We show that HSCs from E11.5 AGM and E12.5 FL efficiently repopulated MCs in recipients. In stark contrast, HSCs from ≥E14.5 FL failed to reconstitute MCs. An Endothelial (EC) fate-mapping study confirmed the EC origin of the majority of MCs. Additionally, our HSC-labeling showed that HSCs do not produce MCs in a physiological setting.

2024年6月1日 · IκBα balances retinoic acid signaling levels together with the epigenetic silencer, PRC2, specifically in HSCs. Loss of IκBα decreases proliferation of HSC and induces a dormancy related gene expression signature instead. Also, IκBα deficient HSCs respond with superior activation to in vitro culture and in serial transplantation.

Recent research revealed that HSCs emerge through multistep maturation of precursors: proHSC → preHSC I → preHSC II → definitive HSC (dHSC). A hallmark of the HSC emergence is the appearance of intra-aortic haematopoietic clusters that are considered to be sites of haematopoiesis.

One day prior to mass emergence of haematopoietic stem cells (HSCs) in the foetal liver at E12.5, the embryo contains only a few definitive HSCs. It is thought that the burst of HSC activity in the foetal liver is underpinned by rapid maturation of immature embryonic precursors of definitive HSCs, termed pre-HSCs.

2016年5月20日 · HSC来源于血管内皮细胞，经过中间状态即造血干细胞前体（pre-HSC）后，最终发育为移植有效的HSC。pre-HSC在胚胎细胞中所占的比例极小，15万胚胎细胞中只有10到20个，而且缺乏特异性的表面标志物将pre-HSC 与其他细胞区分开来，所以一直以来，分离pre-HSC以 …

Definitive hematopoietic stem cells (HSCs) develop in the aorta gonad mesonephros (AGM) region in a stepwise manner. Type I pre-HSCs express CD41 but lack CD45 expression, which is subsequently...

2024年3月16日 · 该研究深入认识了Pre-HSC成熟的精准调控机制，为HSC体外再生的研究提供了重要的理论依据。 造血干细胞（Hematopoietic stem cell, HSC）处于造血谱系的顶端，具有自我更新和多谱系分化能力，能贡献整个生命体的血液系统。

To identify novel secreted regulators of HSC maturation, we performed RNA sequencing over these spatiotemporal transitions in the AGM region and supportive OP9 cell line. Screening several proteins through an ex vivo reaggregate culture system, we identify BMPER as a novel positive regulator of HSC development.