Download and complete this form to establish a PRFA with a state or local government. Download and complete this form to amend an existing PRFA with either a federal or non-federal government agency. The sample Statement of Work (SOW) lists specific removal actions that are ordered through PRFAs.

2017年8月15日 · The Pollution Removal Funding Authorization (PRFA) is a tool available to FOSCs to quickly obtain needed services and assistance from other government agencies: federal, state, or local, as well as recognized Indian Tribes in oil spill and hazardous materials response actions.

Hainzl et al. structurally explain the mechanism of promiscuous (sequence-independent) inhibition of Listeria virulence activator PrfA by environmental oligopeptides. Parallel and antiparallel β sheet-like interactions mediate nonspecific peptide docking at the binding site of the PrfA-activating cofactor, glutathione (GSH).

2024年12月1日 · Positive Regulatory Factor A (PrfA) is a well-studied transcription factor in L. monocytogenes that is responsible for activating a plethora of virulence factors. Targeting virulence factors is a promising strategy that is being considered to combat bacterial infections, hence targeting PrfA is both logical and attractive.

2011年4月1日 · PrfA, a regulatory protein, integrates a number of environmental cues that signal the transition between these two contrasting lifestyles, activating a set of key virulence factors during host infection. While a number of details concerning the general mode of action of this virulence master switch have been elucidated, others remain unsolved.

The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene. [1] In Listeria monocytogenes, virulence genes are maximally expressed at 37 °C (human body temperature) but are almost silent at 30 °C. [2] The genes are controlled by PrfA, a transcriptional activator whose expression is thermoregulated. [3]

2007年8月1日 · The PrfA protein, a member of the Crp/Cap-Fnr family of bacterial transcription factors, controls the expression of key virulence determinants of the facultative intracellular pathogen Listeria monocytogenes. Each of the steps of the listerial intracellular infection cycle—host cell invasion, phagosomal escape, cytosolic replication, and ...

2020年12月8日 · PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors. The temperature-regulated expression of PrfA by its RNAT element serves as a signal of successful host invasion for the bacteria.

2025年1月6日 · PrfA is a key virulence regulator for Listeria monocytogenes (Lm) responding to host environment. Here we report that the natural mutation in PrfA K10N/T151A enhanced the pathogenicity of hypervirulent serotype 4h L. monocytogenes.

单核细胞增生李斯特氏菌(Listeria monocytogenes,简称LM) 是WHO公布的四大食源性致病菌之一,能造成人畜共患的李斯特氏菌病,属于兼性厌氧细胞内寄生革兰氏阳性菌,具有重要的食品卫生安全和医学研究价值。绝大多数LM毒力基因的转录激活受到PrfA蛋白调控。本项目将 ...