Download and complete this form to establish a PRFA with a state or local government. Download and complete this form to amend an existing PRFA with either a federal or non-federal government agency. The sample Statement of Work (SOW) lists specific removal actions that are ordered through PRFAs.

Aug 15, 2017 · The Pollution Removal Funding Authorization (PRFA) is a tool available to FOSCs to quickly obtain needed services and assistance from other government agencies: federal, state, or local, as well as recognized Indian Tribes in oil spill and hazardous materials response actions.

During a response, the FOSC frequently issues Pollution Removal Funding Authorizations (PRFAs) supported by funding from the OSLTF to obtain needed response assistance from other Government agencies (Federal, State, Tribal, or local), such as DOI and its bureaus.

Apr 1, 2011 · PrfA, a regulatory protein, integrates a number of environmental cues that signal the transition between these two contrasting lifestyles, activating a set of key virulence factors during host infection. While a number of details concerning the general mode of action of this virulence master switch have been elucidated, others remain unsolved.

Feb 25, 2025 · Hainzl et al. structurally explain the mechanism of promiscuous (sequence-independent) inhibition of Listeria virulence activator PrfA by environmental oligopeptides. Parallel and antiparallel β sheet-like interactions mediate nonspecific peptide docking at the binding site of the PrfA-activating cofactor, glutathione (GSH).

Jul 10, 2018 · A. Use a Pollution Removal Funding Authorization (PRFA) to request funds from the Oil Spill Liability Trust Fund (OSLTF) to respond to oil discharges. It supplements 264 FW 3, Reimbursable Agreements for Spill Response Activities.

Dec 8, 2020 · PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors. The temperature-regulated expression of PrfA by its RNAT element serves as a signal of successful host invasion for the bacteria.

Aug 27, 2015 · Using a mutant PrfA regulator locked ON (PrfA *) and thus causing PrfA ‐controlled genes to be constitutively activated, we show that virulence gene expression significantly impairs the listerial growth rate (μ) and maximum growth (A) in rich medium.

Aug 1, 2007 · PrfA integrates a number of environmental and bacteria-derived signals to ensure the correct spatio-termporal and niche-adapted expression of the regulon, with maximum induction in the host cell cytosol and repression in the environmental habitat.

Jul 17, 2020 · The PrfA protein, a member of the Crp/Cap-Fnr family of bacterial transcription factors, controls the expression of key virulence determinants of the facultative intracellular pathogen Listeria monocytogenes.