2018年2月14日 · The main difference between probe and primer is that probe is that probe is used to detect the presence of a specific DNA fragment in the mixture through the …

Review options for custom-synthesized oligos, primers, probes, and genes for PCR, cloning, hybridization, real-time PCR, protein expression. Also, access our easy oligo ordering portal or …

2013年10月21日 · From ideal GC content to annealing temperature, here’s everything you need to know about primer and probe design. Many factors can influence PCR experiments, …

Primer and probe are two essential components used in molecular biology techniques like PCR (Polymerase Chain Reaction) and qPCR (quantitative PCR). Primers are short DNA …

Learn about PCR primer and probe chemistries for real-time PCR. Discover DNA primers with fluorescent DNA-binding dyes, hydrolysis probes and molecular beacons.

2024年4月18日 · Probe is a molecule used to detect or measure other substances, often used in diagnostics, while a primer is a short nucleic acid sequence that initiates DNA synthesis in …

2024年6月2日 · Primers are designed to be complementary to particular regions of template DNA, allowing them to bind and serve as a starting point for DNA synthesis. Typically, a pair of …

Design your primers to span an exon-exon junction if you are assessing gene expression in eukaryotic cells to avoid amplifying any contaminating genomic DNA that may

This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. You can customize the potential PCR amplicon's size range, Tm (melting …

Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification …