Compute pI/Mw is a tool which allows the computation of the theoretical isoelectric point (pI) and molecular weight (Mw) of a list of UniProtKB entries or user entered sequences.

This tool calculates the estimated pI and Mw of a specified Swiss-Prot/TrEMBL entry or a user-entered AA sequence. These parameters are useful if you want to know the approximate region of a 2-D gel where a protein may be found. Using Compute pI / Mw

ProtParam [Documentation / Reference] is a tool which allows the computation of various physical and chemical parameters for a given protein stored in UniProtKB or for a user entered protein sequence.

Fig 2: Sample output from the Compute pI / MW tool, where the program was requested to calculate the theoretical pI and MW for the Swiss-Prot entry LACB_BOVIN . Note that the Compute pI / MW tool shows the sequence of the region of the protein that is under consideration.

%PDF-1.4 %âãÏÓ 131 0 obj /Linearized 1 /L 2187060 /H [ 773 607 ] /O 134 /E 4961 /N 38 /T 2184315 >> endobj xref 131 15 0000000016 00000 n 0000000672 00000 n 0000000773 00000 n 0000001380 00000 n 0000001518 00000 n 0000001702 00000 n 0000004078 00000 n 0000004176 00000 n 0000004271 00000 n 0000004363 00000 n 0000004456 00000 n …

The parameters computed by ProtParam include the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient, estimated half-life, instability index, aliphatic index and grand average of hydropathicity (GRAVY).

Compute pI/Mw - References Bjellqvist, B.,Hughes, G.J., Pasquali, Ch., Paquet, N., Ravier, F., Sanchez, J.-Ch., Frutiger, S. & Hochstrasser, D.F. The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences.

ProtScale allows you to compute and represent the profile produced by any amino acid scale on a selected protein. An amino acid scale is defined by a numerical value assigned to each type of amino acid.

Protein-protein interaction networks and enrichment analysis Genes & Genomes , Genomics , Transcriptomics Evolution & Phylogeny ... Compute pI/MW Isoelectric point and molecular weight from protein sequence Proteins & Proteomes Software tool ProtScale Profile produced by amino acids scales on protein sequences ...

PeptideMass [Documentation / References] cleaves a protein sequence from the UniProt Knowledgebase (Swiss-Prot and TrEMBL) or a user-entered protein sequence with a chosen enzyme, and computes the masses of the generated peptides. The tool also returns theoretical isoelectric point and mass values for the protein of interest.