2020年4月23日 · Here, we show that GCN1 is involved in GCN2-mediated eIF2α phosphorylation after AAS and UV radiation by generating Gcn1 mutant mice. Interestingly, GCN1 not only regulates the eIF2α-mediated stress response but also the cell cycle and cell proliferation in a GCN2-independent manner.

2024年8月27日 · Mechanistically, we find that phosphoserine aminotransferase 1 (PSAT1) has a unique capacity for sustained α-KG production when glutamate is depleted. Breast cancer cells with resistance to...

2024年4月1日 · Studies indicate that GCN1 physically interacts with several other proteins involved in stress response. For one, GCN1 recruits the CCR4/NOT nuclease complex to degrade mRNAs with stalled ribosomes (124) (Fig. 2 B). GCN1 also recruits the E3 ubiquitin ligase, RNF14, to help degrade the elongation factor eEF1A on stalled ribosomes (125). In ...

2023年6月25日 · 在一项新的研究中，来自德国马克斯-普朗克生物化学研究所的研究人员如今发现了一种能够启动有缺陷的蛋白的靶向降解的新机制。 蛋白GCN1在这一过程中是至关重要的。 相关研究结果于2023年6月19日在线发表在Cell期刊上，论文标题为“Mechanisms of readthrough mitigation reveal principles of GCN1-mediated translational quality control”。 核糖体是制造我们细胞中所有蛋白的分子机器。 有机体的遗传密码经转录后产生所谓的信使RNA（mRNA）。 核 …

In this study, we demonstrate that phosphoserine aminotransferase 1 (Psat1), an Oct4/Sox2/Nanog (OSN) target protein, regulates changes in α-ketoglutarate (α-KG), determining the fate of mouse ESCs (mESCs). Maintaining Psat1 levels was essential for mESC self-renewal and pluripotency.

2022年10月10日 · 该研究发现在肺腺癌细胞经Erlotinib短期适应性耐药和长期获得性耐药过程中，葡萄糖来源从头合成的丝氨酸及其合成通路关键酶PHGDH、PSAT1和PSPH蛋白水平均显著增加。 敲低PSAT1能优先抑制耐药细胞的增殖及运动，恢复耐药细胞对Erlotinib的敏感性，增强Erlotinib对肺腺癌的杀伤作用。 反之过表达PSAT1可促进肺腺癌Erlotinib耐药和肿瘤转移。 机制研究表明PSAT1不仅通过丝氨酸合成通路调控肺腺癌细胞内氧化还原稳态，还能不依赖于其经典 …

Mechanistically, we find that phosphoserine aminotransferase 1 (PSAT1) has a unique capacity for sustained α-KG production when glutamate is depleted. Breast cancer cells with resistance to...

2023年6月19日 · Selective ribosome profiling revealed a general function of GCN1 in translational surveillance, with GCN1 preferentially recognizing ribosome slowdown and collision at nonoptimal codons in hydrophobic 3′ UTRs, transmembrane proteins, and collagens (Figures 7A and 7B), thus defining GCN1 as a key regulator of translational dynamics, a function ...

2024年6月1日 · The expression levels of GCN1 in serum exhibit a correlation with radon exposure, positioning GCN1 as a promising early molecular biomarker for radon-induced LC. Furthermore, pathogenic mutations within GCN1 have been identified in at least two female patients with lung adenocarcinoma.

2022年10月4日 · Here, we found that aberrant upregulation of phosphoserine aminotransferase 1 (PSAT1) confers erlotinib resistance and tumor metastasis in lung adenocarcinoma. Depletion of PSAT1 restored sensitivity to erlotinib and synergistically augmented the tumoricidal effect.