Alpha-kinase 1 is a cytosolic innate immune receptor for ... - Nature
2018年8月15日 · The Himar1 mariner transposon vector pSC123 33 (kindly provided by A. Rietsch, Case Western Reserve University and S. Lory, Harvard Medical School) was used to generate the mutant library of Y ...
DNASU Plasmid | Detailed Vector Information: pSC123
A repository of over 200,000 plasmids including Protein Structure Initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms.
PNP降解相关基因温敏突变株的分离及其特性研究
通过接合转移将质粒pSC123上的转座子Tn5随机插入到DLL-E4基因组DNA中,从大约8,000个突变株中得到1株温度敏感型突变株MT54。 根据转座子上的已知序列设计引物以MT54基因组DNA为模板进行PCR扩增,证实MT54的染色体中有转座子插入。 MT54在30℃条件下能够以对硝基苯酚 (p -nitrophenol,PNP)为唯一碳源生长,但在37℃不能生长。 格里斯试色剂法检测NO 2 -的生成情况进一步证明了MT54的这种特性。 通过30℃和37℃两种温度条件下MT54和原始出发菌株DLL-E4 …
Identification of two combined genes responsible for …
2013年9月15日 · Random mutagenesis of strain P2 (ampicillin resistant) was accomplished by the introduction of a plasmid vector pSC123 [20], which carries a pMB1-based replication machinery in a Tn5-derived mini-transposon and harbors a kanamycin resistance gene for selecting transposon insertions.
Flagellin-Deficient Legionella Mutants Evade Caspase-1- and
2006年3月17日 · Macrophages from C57BL/6J (B6) mice restrict growth of the intracellular bacterial pathogen Legionella pneumophila. Restriction of bacterial growth requires caspase-1 and the leucine-rich repeat-containing protein Naip5 (Birc1e). We identified mutants of L. pneumophila that evade macrophage innate immunity.
The Novel Bacterial N-Demethylase PdmAB Is Responsible for the …
Sphingomonas sp. strain SRS2, isolated from IPU-treated agricultural soil in the United Kingdom, is the first bacterium described as being able to mineralize the phenyl structure of a phenylurea herbicide. Strain SRS2 was able to not only mineralize IPU but also transform other N, N -dimethyl-substituted phenylurea herbicides (17, 33).
Rationally designed mariner vectors for functional genomic analysis …
2021年11月26日 · Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS.
psc123 mariner transposon 水手转座子 - 转座子质粒系统 …
psc123 mariner transposon 水手转座子: 发布时间:2022-03-16 15:31:10 | 浏览次数： psc123 mariner transposon 水手转座子。可以用来构建细菌转座子突变文库。 上一篇:pEP4351 转座子质粒 下一篇:mariner转座子质粒pFAC
求助pSC123质粒 - 丁香园论坛 - DXY.cn
pSC123质粒含有Himar1转座子，所以特来站里向各位战友求助，有该质粒的战友可以跟我交换其他的克隆或表达质粒，或细胞系都是可以的，小弟不胜感激！
pSC123含Tn5转座子的自杀性质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
您正在向 biovector.net 发送关于产品 pSC123含Tn5转座子的自杀性质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问. 点击“立即发送”后，我们将在1个工作日内与您取得联系。 订单付款. Order & Payment.