2019年3月29日 · Of the four separate PE biosynthetic pathways in eukaryotes, one occurs in the mitochondrial inner membrane (IM) and is executed by phosphatidylserine decarboxylase (Psd1). Deletion of Psd1...

2018年1月22日 · We examined the roles of Psd1 ER and/or Psd1 mito in extra-mitochondrial cellular PE homeostasis. In the absence of Psd2 and Dpl1, Psd1 is the only source of cellular PE and is thus essential for cell viability unless growth medium is supplemented with exogenous ethanolamine to fuel PE production via the Kennedy pathway (Figure 3F).

2012年11月23日 · Here, we show that mitochondrial-localized Psd1, the key yeast enzyme that synthesizes PE, is required for proper mitochondrial morphology and fusion. Yeast cells lacking Psd1 exhibit fragmented and aggregated mitochondria with …

2017年1月1日 · Phosphatidylserine decarboxylases (PSD) form phosphatidylethanolamine. Type I PSDs are mitochondrial, whereas other PSDs are extramitochondrial components. We describe function, enzymology, biogenesis and assembly of Psd1 into mitochondria. Contributions of PSDs to lipid homeostasis and their cellular physiology are discussed.

Here, we show that the abundance of phosphatidylethanolamine (PE) positively regulates autophagy. Reduction of intracellular PE levels by knocking out either of the two yeast phosphatidylserine...

磷脂酰丝氨酸脱羧酶 1 (Psd1) 通过调节线粒体膜的生物物理特性和线粒体基因组维持蛋白 1 (Mgm1) 的替代拓扑生成来促进线粒体融合。 Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada. 背景：磷脂酰乙醇胺被提议用于调节线粒体融合，但其作用机制尚不清楚。 结果：减少磷脂酰乙醇胺会降低脂质混合率和线粒体融合蛋白 Mgm1 的生物发生。 结论：Psd1 调节线粒体融合的脂质和蛋白质机制。 意义：了解脂质代谢如何调节线粒体动力 …

2022年5月1日 · 我们现在解决了这个谜团，并证明 ER 定位的 Psd1 是 ER 子域脂滴 (LD) 生物发生的关键调节因子。 虽然它组成性地靶向 ER 膜，Psd1 瞬时集中在 ER 到 LD 附着位点，特别是在受刺激的 LD 生物发生期间。 使用诱导型 LD 生物发生测定，我们证明 ER 定位的 Psd1 是正常 LD 形成所必需的。 此外，我们确定了 Psd1 上的 LD 结合基序，并表明该基序是 Psd1 影响 LD 形态发生所必需的。 我们还发现，磷脂酰丝氨酸脱羧酶 (PSD) 酶在 LD 形成中的作用在裂殖酵母 …

2015年1月9日 · Here, we show that the abundance of phosphatidylethanolamine (PE) positively regulates autophagy. Reduction of intracellular PE levels by knocking out either of the two yeast phosphatidylserine...

Recently, the strict mitochondrial localization of the enzyme that makes PE in the mitochondrion, phosphatidylserine decarboxy-lase 1 (Psd1), was questioned. Since a dual localization of Psd1 to the ER would have far-reaching implications, we initiated our study to independently re-assess the subcellular distribution of Psd1.

2024年7月24日 · PE synthesis occurs via two major routes: the CDP-ethanolamine (Etn) Kennedy pathway in the endoplasmic reticulum (ER) and via phosphatidylserine decarboxylase (Psd1/PISD) in the inner mitochondrial membrane (IMM). Suggestive of dual requirement, total loss of either major PE synthesis pathway is embryonically lethal in mammals.