2021年8月2日 · These results indicate that rHMGB1 promotes NRF2 independent mitochondrial fusion via CXCR4/PSMB5 pathway-mediated Drp1 proteolysis. rHMGB1 may influence mitochondrial and endothelial function through this effect on mitochondrial dynamics.

2021年8月2日 · Schematic depiction of rHMGB1-induced mitochondrial fusion and the underlying mechanism. rHMGB1 combines and activates the membrane receptor CXCR4, then upregulates the expression of PSMB5 to increase the activity of 20S proteasome, leads to degradation of mitochondrial fission protein Drp1, and results in mitochondrial hyperfusion. rHMGB1 ...

Proteasome beta 5 (PSMB5), which can degrade many aberrant and denatured intracellular proteins as well as functional proteins, is mediated by the Nrf2-ARE pathway in many cell types.

2021年6月28日 · Proteasome gene expression is triggered by the nuclear respiratory factor 1 (Nrf1). This transcription factor is, in turn, regulated by the mammalian target of rapamycin complex 1 (mTORC1) and a feedback mechanism compensating proteasome dysfunction .

2006年7月14日 · Activation of the Psmb5 promoter through the Nrf2-ARE pathway following 3-MC incubation is demonstrating that this system can be regulated by environmental stimuli. Dynamic changes of proteasome expression upon exposure to exogenous chemicals may be an important part of the cellular adaptive response against oxidative stress to promote cell ...

Murine PSMB5 promoter constructs (A) and luciferase activities derived from these truncated promoters following treatment with sulforaphane (10 M) or cotransfection of an Nrf2 expression plasmid...

2024年12月25日 · PSMB5 (Proteasome 20S Subunit Beta 5) is a Protein Coding gene. Diseases associated with PSMB5 include Cystic Fibrosis and Parkinson's Disease. Among its related pathways are Autodegradation of the E3 ubiquitin ligase COP1 and CDK-mediated phosphorylation and removal of Cdc6.

患者基因检测结果，我们发现6例（2.1%）患者在耐 药后出现了PSMB5基因突变，其中4例患者获得了 部分缓解（partial response，PR）疗效，2例患者疗 效为疾病稳定（stable disease，SD）。上述6 例 PSMB5突变中，4例为p.A79T，2例为p.M104I。 1.2 DNA/RNA提取与测序

ARE-containing promoter regions from PSMB5 and the GSTA1 promoter were detected in Nrf2 immunoprecipitants obtained from sulforaphane-treated wild-type cells.

These results demonstrated that IA could promote PSMB5 expression in an Nrf2-dependent manner, resulting in the suppression of mitochondrial fission, and thus improve endothelial dysfunction. These findings laid a foundation to the future development of IA as an agent to the prevention and treatment of AS.

2021年2月1日 · Immunoproteasome expression can be increased by interferon regulatory factor 1 (IRF1) after its expression is upregulated by the interferon gamma (IFNγ)-induced Stat1 transcription factor (left panel). Upregulation of the PSMB 1–7 genes by epidermal growth factor (EGF)-induced STAT3 can play a role in oncogenic proliferation (right panel ...

2006年7月14日 · Collectively, these results indicate that expression of proteasome subunit PSMB5 is modulated by bifunctional enzyme inducers in a manner independent of the AhR/Arnt-XRE pathway but dependent upon the Nrf2-ARE pathway.

2021年8月3日 · These results indicate that rHMGB1 promotes NRF2 independent mitochondrial fusion via CXCR4/PSMB5 pathway-mediated Drp1 proteolysis. rHMGB1 may influence mitochondrial and endothelial function...

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(A) Luciferase activity driven by the proximal PSMB5 promoter ( Ϫ 1.1kb-luc) following treatment with antioxidants in wild-type and nrf2 -disrupted cells. BHT, butylhydroxytoluene; EQ,...

2017年11月1日 · Based on our bioinformatics analysis, we hypothesized that PSMB5 contained immunosuppressive and oncogenic characteristics. To study the effects of PSMB5 on the cancer cell and macrophage in vitro, we silenced PSMB5 expression with shRNA in THP-1 monocytes and MDA-MB-231 cells respectively.

2008年9月15日 · Study of the molecular mechanism of bortezomib resistance in these cells revealed (1) an Ala49Thr mutation residing in a highly conserved bortezomib-binding pocket in the proteasome β5-subunit (PSMB5) protein, (2) a dramatic overexpression (up to 60-fold) of PSMB5 protein but not of other proteasome subunits including PSMB6, PSMB7, and PSMA7 ...

psmb5参与抗原呈递和氧化应激，与b细胞、cd4+t细胞、cd8+t细胞、树突状细胞、巨噬细胞和中性粒细胞的丰度密切相关。psmb5可作为hcc诊断和预后的生物标志物。此外，psmb5在多种癌症中起促癌作用，包括乳腺癌、食管鳞状细胞癌和前列腺癌。

Depleting PSMB6 prevented splicing of the major catalytic subunits PSMB5, PSMB7, PSMB8, and PSMB10; however, PSMB6 engineered without splicing function or catalytic activity, also restored viability, inferring the contribution of PSMB6 to proteasome structure to be more important than functional activity.

A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.