May 8, 2023 · Here we describe a novel narnavirus, Puccinia striiformis virus 5 (PsV5), from the devastating wheat stripe rust fungus P. striiformis f. sp. tritici (Pst). The genome of PsV5 contains two predicted open reading frames (ORFs) that largely overlap on reverse strands: an RNA-dependent RNA polymerase ( …

May 8, 2023 · In this study, we report a novel ambigrammatic narnavirus, P. striiformis virus 5 (PsV5), from Pst. We demonstrate the “two-way” coding phenomenon of PsV5 and the positive effect of PsV5 on Pst pathogenesis.

May 8, 2023 · PsV5的基因组包含两个预测的 开放阅读框 （ORF），它们在反向链上大量重叠：一个是RNA依赖性RNA聚合酶（RdRp），另一个是功能未知的反向阅读框（rORF）。 免疫技术证实了这两种ORF的蛋白质翻译。 过表达PsV5（RdRp-rORF）、RdRp-ORF或rORF的转基因小麦系更容易受到Pst感染，而PsV5 RNA干扰（RNAi）系更具抗性。 PsV5（RdRp-rORF）、RdRp-ORF或rORF在 禾谷镰刀菌 中的过表达也增强了真菌的毒力。 因此，我们报道了一种新 …

采用HIGS技术沉默CYR31中的病毒PsV5，可以显著降低锈菌的毒力，在小麦中过表达病毒或正、反向编码蛋白，都可以使得小麦对锈菌更敏感，这个结果表明PsV5基因组编码的RdRp和rORF蛋白在小麦-条锈菌互作系统中正调控锈菌毒力。

Feb 17, 2025 · A novel ambigrammatic mycovirus PsV5 works hand in glove with wheat stripe rust fungus to facilitate infection. Plant communications, 2022, 100505. (2022, TOP, IF=10.5)

ambigrammatic narnavirus, P. striiformis virus 5 (PsV5), from Pst. We demonstrate the ‘‘two-way’’ coding phenomenon of PsV5 and the positive effect of PsV5 on Pst pathogenesis. This study not only provides a deeper understanding of the biology of +ssRNA viruses but also identifies a mycovirus target with disease engineering potential ...

Oct 1, 2023 · This paper showed how an ambinarnavirus PsV5 affects the virulence of a host obligate biotroph Pst, which is pathogenic to wheat. The authors indirectly showed that PsV5 enhances Pst virulence using wheat transgenic plants expressing PsV5 siRNAs or genes.

PsV5促进其真菌宿主毒力的事实可用于开发防治小麦条锈病的新策略。 研究人员认为，RNAi技术可以用于靶向病毒，减少其对小麦作物的影响。 总的来说，这项研究为致力于防治植物疾病和提高全球粮食安全的科学家提供了有价值的信息。

Oct 1, 2023 · A new example is an ‘ambinarnavirus’ termed Puccinia striiformis virus 5 (PsV5), which enhances the virulence of a destructive phytopathogenic basidiomycete, Puccinia striiformis f. sp. Tritici (Pst), that is the causal agent of wheat stripe rust [49]. Unlike many other narnaviruses with simple (+)ssRNA genomes

May 8, 2023 · This study reports the identification of a novel ambigrammatically encoded naked RNA virus, Puccinia striiformis virus 5 (PsV5), in wheat stripe rust. Using immune technology, the RdRp ORF and rORF of PsV5 were confirmed to encode proteins, and using transgenic technology, the presence of PsV5 was shown to enhance the virulence of the rust fungus.