Staphylococcus aureus plasmid pUB110 replicates in B. subtilis. Transfer between Bacillus species by transformation or transduction using bacteriophages PBS1, AR9, or Sp10. A natural isolate general purpose plasmid vector for cloning in Bacillus. [4] Medium is 1179 V Y medium. Hosts: Staphylococcus aureus, B.subtilis BD170, B.subtilis.

Plasmid pGL0_9 [pUB110 ORI] from Dr. Nili Ostrov's lab contains the insert pUB110 ORI and is published in bioRxiv 2023.05.19.541510 This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format

来源于金黄色葡萄球菌的质粒pC194、pE194、pT181和pUB110 均是以滚环复制方式进行复制的。 暋暋pC194 大小为2906bp,抗 氯霉素特性,质粒的拷贝数约15 个染色体[6],该 质粒较小,容 易克隆,当插入外源片段时容易产生大分子的畸形复制产物,从而高度不稳定,这就决定了该质粒载体在宿主中的应用性偏低;pE194 大小为3728bp,温度敏感型质粒载体,拷 贝数大约为10 个染色体,该质粒载体在32曟 正常复制,随 着温度的升高拷贝数会逐渐降低,达到45曟 时停止拷贝,所以在枯草芽孢杆 …

general purpose plasmid vector for cloning in Bacillus. ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

pUB110载体价格3800元，pUB110载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为卡那，质粒大小为4.6kb。pUB110载体是质粒。

General purpose plasmid vector for cloning in Bacillus. Sequence known. Copy number in B. subtilis about 50. The pUB110 replicon is the basis for the construction of chimeric plasmid vectors.

pBE-S DNA载体含有能够在B. subtilis 中发挥作用的pUB110来源的复制起始位点(pUB ori)和一个卡那霉素抗性基因(Kan r)，以及能在E.coli 中发挥作用的pUC来源的复制起始位点(CoIE ori)和一个氨苄青霉素抗性基因(Amp r)。

来源于金黄色葡萄球菌的天然质粒，多用于芽孢杆菌克隆表达质粒构建，Copy number in B. subtilis about 50.

（1） 克隆菌株：pUB110 需要在枯草芽孢杆菌中扩增和复制。 （2） 原核抗性：Kan+，工作浓度建议为25ug/ml。 （3） 培养温度：37℃。 （4） 拷贝数（Copy number）：极低。pUB110 质粒拷贝数非常低，常规小提很难提到质粒，建议用大 提试剂盒提取质粒。

2021年5月8日 · Two series of pUB110-derived plasmids were constructed to study segregational stability in Bacillus subtilis. pEB plasmids were based on the entire pUB110, whereas pLB plasmids lack the membrane-binding areas BA3 and BA4. Two kinds of stability defects were observed. The first was characterized by a …