2020年1月6日 · Potato virus Y (PVY) is a major pathogen of potatoes with major impact on global agricultural production. Resistance to PVY can be achieved by engineering potatoes to …

2022年8月26日 · To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for …

2021年9月13日 · The purpose of this work was (i) to establish what eIF4E isoforms interact with VPg of the PVY strain NTN (VPg-NTN), one of the most infectious and widespread PVY …

2022年7月12日 · Mutated eIF4E lines showed enhanced resistance to PVY O strain. DAS-ELISA and RT-PCR were used for validation of the observed resistance. PVY resistance in tetraploid …

2024年1月8日 · In this study, we found that the single amino acid substitution D71G in the cap-binding pocket of eIF4E is sufficient to confer resistance to PRSV-W and ZYMV in watermelon …

Here, we showed that the most common VPg variant from the PVY strain NTN interacts with eIF4E1 and eIF4E2, but not with eIF(iso)4E. Based on the VPg, eIF4E1, and eIF4E2 models …

2024年4月1日 · A necessary condition for PVY infection is an interaction between the plant cap-binding translation initiation factors eIF4E and a viral protein VPg, which mimics the cap …

The interaction between the viral protein genome-linked (VPg) and eukaryotic initiation factor 4E (eIF4E) or eIF(iso)4E of the host plays a crucial role in potyvirus infection. The VPg of potato …

2019年12月12日 · This study is a proof-of-concept for successful generation of viral resistance in potato by the RNAi-mediated transcriptional regulation of targeted eIF4E gene family using …

To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene …