2020年1月6日 · Potato virus Y (PVY) is a major pathogen of potatoes with major impact on global agricultural production. Resistance to PVY can be achieved by engineering potatoes to express a recessive, resistant allele of eukaryotic translation initiation factor eIF4E, a host dependency factor essential to PVY replication.

2022年8月26日 · To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing...

2021年9月13日 · The purpose of this work was (i) to establish what eIF4E isoforms interact with VPg of the PVY strain NTN (VPg-NTN), one of the most infectious and widespread PVY strains, (ii) to identify amino acid residues crucial for this interaction, and (iii) to interpret the obtained data form the structural point of view in order to elucidate the ...

2022年7月12日 · Mutated eIF4E lines showed enhanced resistance to PVY O strain. DAS-ELISA and RT-PCR were used for validation of the observed resistance. PVY resistance in tetraploid lines via CRISPR/Cas9 provides a route to develop novel resistant potato cultivars.

2024年1月8日 · In this study, we found that the single amino acid substitution D71G in the cap-binding pocket of eIF4E is sufficient to confer resistance to PRSV-W and ZYMV in watermelon or PVY in tobacco. Moreover, the PI 244019 male flower development was normal and could produce fruits through self-pollination (Figure S5 ), suggesting the amino acid ...

Here, we showed that the most common VPg variant from the PVY strain NTN interacts with eIF4E1 and eIF4E2, but not with eIF(iso)4E. Based on the VPg, eIF4E1, and eIF4E2 models and data on the natural polymorphism of VPg amino acid sequence, we suggested that the key role in the recognition of potato cap-binding factors belongs to the R104 ...

2024年4月1日 · A necessary condition for PVY infection is an interaction between the plant cap-binding translation initiation factors eIF4E and a viral protein VPg, which mimics the cap-structure. In this study, we identified the point mutations in potato eIF4E1 and eIF4E2 that disrupt VPg binding while preserving the functional activity.

The interaction between the viral protein genome-linked (VPg) and eukaryotic initiation factor 4E (eIF4E) or eIF(iso)4E of the host plays a crucial role in potyvirus infection. The VPg of potato virus A (PVA) contains the Tyr-X-X-X-X-Leu-phi ...

2019年12月12日 · This study is a proof-of-concept for successful generation of viral resistance in potato by the RNAi-mediated transcriptional regulation of targeted eIF4E gene family using intragenic approach.

To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing of two clades, eIF4E1 () and eIF4E2 () in the gene family comprising six …