2010年11月22日 · Our complex structure reveals a unique type of SH3 interaction based on recognition of tandem PxxP motifs in the ligand. Strikingly, the specificity pocket of IRTKS SH3 has evolved to accommodate a polyproline type II helical peptide analogously to docking of the canonical PxxP by the conserved IRTKS SH3 proline-binding pockets.

SH3 domains bind proline-rich sequences, particularly those carrying the PxxP motif that have the left-handed polyproline 2 (PPII) conformation (Mayer 2001; Musacchio 2002). Two classes of peptide ligands have been identified: class I binds with the consensus sequence RXLPPXP, and class II binds with the consensus sequence XPPLPXR (Feng et al ...

2012年8月14日 · This bacterial peptide contains a typical PPII-helical PxxP motif to interact with the xP pocket region of IRTKS SH3 as a Class I ligand, but exploits a unique strategy for interacting with the specificity zone.

2021年3月12日 · SH3s typically bind to Pro/Arg-rich peptide motifs on their target partners with an archetypical PXXP motif (where X represents any amino acid) 6, 10. In this work, we combine genome editing,...

2024年4月18日 · By combining biochemical methods and structural biology, we show that the C-terminal SH3 domain of PEX13 mediates intramolecular interactions with a proximal FxxxF motif. The SH3 domain also...

2015年4月28日 · We find that a single polyproline (PXXP) motif is sufficient for the SH3 domain-mediated complex formation with all four proteins. Fig. 1. SrGAP3 interacts with SH3 domain-containing proteins in vivo. (A) Domain architecture of srGAP3 full-length and the C-terminal region (CTR) fused to GST.

络氨酸磷酸化是调节接头结构域（adaptor domains）活性的一种重要机制。络氨酸的磷酸化能够促进 信号蛋白 通过SH2或PTB结构域来相互作用，但是SH3结构域的磷酸化作用却不然，它反而妨碍了信号 分子 之间的相互作用。研究人员Marian Novotny表示，这种络氨酸磷酸化 ...

2022年9月1日 · Long described as modular/portable domains targeting proline-rich (PXXP-like) peptide motifs, recent findings challenged this simplistic view by demonstrating that SH3 domains can have more complex roles that are regulated in part allosterically by their host protein context, tyrosine phosphorylation (see Glossary), and phase separation. Here ...

2010年12月14日 · Our complex structure reveals a unique type of SH3 interaction based on recognition of tandem PxxP motifs in the ligand. Strikingly, the specificity pocket of IRTKS SH3 has evolved to accommodate a polyproline type II helical peptide analogously to docking of the canonical PxxP by the conserved IRTKS SH3 proline-binding pockets.

2020年1月1日 · 动机超过一半的人类蛋白质组包含富含脯氨酸的基序pxxp。该基序极有可能采用左旋聚脯氨酸ii（ppii）螺旋，并可能结合sh3域。根据ppii是按正向（n对c末端）还是负向（c对n末端）结合，sh3域通常分为两类。