2021年5月22日 · Among them, the ncNLS of the “proline-tyrosine” category, named PY-NLS was studied in most detail. PY-NLS is characterized by 20–30 amino acids that assume a disordered structure, consisting of N-terminal hydrophobic or basic motifs and C-terminal R/K/H(X) 2-5 PY motifs (where X 2-5 is any sequence of 2–5 residues) [ 27 ].

NPC对蛋白质的运输是双向的，胞浆蛋白入核的标签称为 核定位信号 （nuclear localization signal，NLS），核内蛋白出核的标签称为 核输出信号 （nuclear export signal，NES）。 最初鉴定的NLS多富含碱性氨基酸，所以此类NLS称为碱性NLS或经典NLS（cNLS）。

A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport. [1] Typically, this signal consists of one or more short sequences of positively charged lysines or arginines exposed on the protein surface. [ 1 ]

Here, we demonstrate that the PY-NLS is conserved in Saccharomyces cerevisiae and show for the first time that the PY-NLS is a functional nuclear targeting sequence in vivo. The apparent ortholog of Kapβ2 in yeast, Kap104, has two known cargos, the mRNA-binding proteins Hrp1 and Nab2, which both contain putative PY-NLS-like sequences.

Here, we show that Pab2 contains a proline-tyrosine nuclear localization signal (PY-NLS) that is necessary and sufficient for its nuclear localization and function. Consistent with the role of karyopherin β2 (Kapβ2)-type receptors in the import of PY-NLS cargoes, we show that the fission yeast ortholog of human Kapβ2, Kap104, binds to ...

2023年12月5日 · 核定位信号（nls）主要分为3种类型：（1）经典核定位信号；（2）转运蛋白β2识别的核定位信号，又叫py-nls；（3）核定位信号斑；（4）假核定位信号；（5）其他

2020年12月11日 · Remarkably, the structure reveals that the nuclear localization sequence of hnRNPA1 (termed PY-NLS), which is initially known to mediate the nucleo-cytoplamic transport of hnRNPA1 through...

2008年5月9日 · Here, we demonstrate that the PY-NLS is conserved in Saccharomyces cerevisiae and show for the first time that the PY-NLS is a functional nuclear targeting sequence in vivo. The apparent ortholog of Kapbeta2 in yeast, Kap104, has two known cargos, the mRNA-binding proteins Hrp1 and Nab2, which both contain putative PY-NLS-like sequences.

2012年11月14日 · Chemical or genetic inhibition of arginine methylation restores TRN-mediated nuclear import of ALS-associated FUS mutants. The unmethylated arginine-glycine-glycine domain preceding the PY-NLS interacts with TRN and arginine methylation in …

MP NLS are a single cluster composed of 4–8 basic amino acids, which generally contains 4 or more posi-tively charged residues, that is, arginine (R) or lysine (K). The characteristic motif of MP NLS is usually defined as K (K/R) X (K/R), where X can be any residue [17].