2024年12月10日 · Example: Cas9 • Streptococcus pyogenes Cas9 = SpCas9 (There are various Cas9 proteins from different species; for example, Staphylococcus aureus Cas9, SaCas9) • Single-guide RNA = sgRNA • Target DNA = Target strand + Nontarget strand

2021年6月17日 · Cas9介导的DNA切割最后结果是目标DNA（PAM序列上游约 3～4个核苷酸）的双链断裂（double strand break，DSB）。 上图是切割位置示意图，实验中观察到的切割位点由黑色（HNH）和蓝色（RuvC）三角形标记（蓝色填充:首选位点，无填充:典型位点(较不利)，青色填 …

2014年1月16日 · Cas9 has been harnessed as a powerful tool for genome editing and gene regulation in many eukaryotic organisms. We report 2.6 and 2.2 angstrom resolution crystal structures of two major Cas9 enzyme subtypes, revealing the structural core shared by all Cas9 family members.

2015年10月28日 · Cas9 is an RNA-guided DNA endonuclease that targets foreign DNA for destruction as part of a bacterial adaptive immune system mediated by clustered regularly interspaced short palindromic repeats...

2015年12月10日 · We determine molecular structures of the catalytically active Streptococcus pyogenes Cas9 R-loop that show the displaced DNA strand located near the RuvC nuclease domain active site. These protein-DNA interactions, in turn, position the HNH nuclease domain adjacent to the target DNA strand cleavage site in a conformation essential for concerted ...

2014年1月31日 · The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 Å resolution.

We aimed to use Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) gene editing technique to induce forced down-regulation of the circRNA which consequently modified miRNA...

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2025年2月16日 · After discussion, we decided to make the Cas9 molecule from Streptococcus pyogenes (SpCas9) for the following reasons: First, this molecule is what Xinyin likes and is currently working with. Second, Xinyin had some experience in using PDB and Pymol. Although it seems challenging to make, we thought it should be achievable.

2018年10月3日 · Streptococcus pyogenes Cas9 (SpyCas9) is a 1368-amino acid DNA endonuclease which has two distinct lobes: the alpha-helical recognition lobe (REC), which is responsible for holding the DNA in place, and the nuclease lobe (NUC) containing the HNH and RuvC nuclease domains that snip the target double-stranded DNA (dsDNA).