QD-labeled WT/H144A/Q258A JEV virions were allowed to infect living Vero cells at 37°C and imaged in real time by a spinning-disk confocal microscope. (A, E, and I) Snapshots of QD-labeled ...

2024年7月30日 · Certain residues within the E protein have key functions in the assembly, release, and entry mechanisms of JEV, for example, the H144A, H319A, T410A, and Q258A mutants exhibiting weak fusion ability [13]. In addition, the JEV E protein is involved in humoral immunity, especially E-specific antibodies [14].

2015年4月21日 · T406 in TBEV, equivalent to T410 in JEV, is predicted to form hydrogen bonds with Q260 at the beginning of the aB helix (Q258 in JEV) , but our data show that the entry activity of T410A relative to that of the WT was reduced by only 40%, much less than that with Q258A.

The results showed N154 and the DE loop are not the only or major receptor-binding motifs for JEV entry into BHK-21 cells; four residues, H144, H319, T410, and Q258, participating in the domain I (DI)-DIII interaction or zippering reaction are important to maintain the efficiency of viral membrane fusion.

2015年3月16日 · 中国科学院武汉病毒研究所肖庚富研究员领导的科研团队在乙型脑炎病毒（Japanese encephalitis virus，JEV）囊膜蛋白介导病毒入侵宿主细胞分子机制研究方面取得重要进展。 相关研究结果“Structure-based mutational analysis of several sites in the E protein: Implications for understanding the entry mechanism of Japanese encephalitis virus”近日在病毒学领域重要期刊Journal of virology上在线发表。

中国科学院武汉病毒研究所研究员肖庚富领导的科研团队在乙型脑炎病毒（Japanese encephalitis virus，JEV）囊膜蛋白介导病毒入侵宿主细胞分子机制研究方面取得新进展，相关研究结果Structure-based mutational analysis of several sites in …

2015年3月17日 · 中国科学院武汉病毒研究所研究员肖庚富领导的科研团队在乙型脑炎病毒（Japanese encephalitis virus，JEV）囊膜蛋白介导病毒入侵宿主细胞分子机制研究方面取得新进展，相关研究结果 Structure-based mutational analysis of several sites in the E protein: Implications for understanding the entry mechanism of Japanese encephalitis virus 近日在病毒学期刊Journal of virology 上在线发表。 乙脑病毒是和黄热病毒、登革热病毒、西尼罗病毒同一 …

2015年3月30日 · 近日，中科院武汉病毒研究所研究员肖庚富领导的科研团队在乙型脑炎病毒（Japanese encephalitis virus,JEV）囊膜蛋白介导病毒入侵宿主细胞分子机制研究 ...

Here, we labeled the WT, H144A mutant, and Q258A mutant JEVs with QDs to visually analyze the effect of the two substitutions on virus infection. The nearly overlapping one-step growth curves of...

With the aid of this QD labeling method, we lit wild-type and mutant Japanese encephalitis viruses up, tracked their infection in living Vero cells, and found that H144A and Q258A substitutions in the envelope protein did not affect the virus intracellular trafficking.