Rpb1 CTD (4H8) Antibody detects endogenous levels of total Rpb1 protein (both phosphorylated and unphosphorylated forms). The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has …

人类 RNA聚合酶 (Pol) II 的RPB1亚基的C 端结构域 (CTD) 的过度磷酸化对于转录延伸和 mRNA 加工至关重要1–3。 CTD包含52个共有序列YSPTSPS的七肽重复序列。 C TD的高度重复性和丰富的可能磷酸化位点对催化其过度磷酸化的激酶具有特殊的限制作用。 正转录延伸因b (P-TEFb)，由 CDK9 和 细胞周期蛋白T1 组成已知可使CTD和负延伸因子过度磷酸化以刺激Pol II延长 1,4,5。 P-TEFb上促进这一作用的序列决定簇目前尚不清楚。 研究结果. 图1：通过亲和纯化 …

The C-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (RNApII), coordinates recruitment of RNA- and chromatin-modifying factors to transcription complexes. It is unclear whether the CTD communicates with the catalytic core ...

Phospho-Rpb1 CTD (Ser2/5) Antibody detects endogenous levels of Rpb1 when the carboxy-termial domain heptapeptide repeat [Tyr1,Ser2,Pro3,Thr4,Ser5,Pro6,Ser7] is dually phosphorylated at Ser2 and Ser5 and singly phosphorylated at either Ser2 or Ser5.

A distinctive feature of Pol II is the carboxy-terminal domain (CTD) of its largest subunit, RPB1, which is composed of a series of heptapeptide repeats that play a vital role in transcription. Here, we provide a comprehensive review and summary of the sequence characteristics and evolutionary trajectory of the eukaryotic RPB1 CTD, as well as ...

2018年10月18日 · Co-transcriptional tethering of the 5′ exon may involve the flexible C-terminal domain (CTD) of Pol II’s largest subunit, Rbp1, which acts to coordinate transcription with pre-mRNA processing (Heidemann et al., 2013, Zaborowska et al., 2016). Mammalian CTD comprises 52 repeats of a heptad with the consensus amino acid sequence YSPTSPS.

2014年5月23日 · The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II), RBP1, consists of repeats of the sequence YSPTSPS. Post-transcriptional...

2016年3月22日 · We prepared polyclonal antibodies to YSATLRY from human sera to immunoprecipitate the native antigen, which was identified as the carboxy-terminal domain (CTD) of DNA-directed RNA polymerase II subunit RPB1. The RBP1 CTD contains multiple YSPTSPS repeats, which are significantly homologous to YSATLRY and YSPTLFY.

The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y1S2P3T4S5P6S7. We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3′-end ...

Post-transcriptional modifications at specific CTD residues are crucial for transcriptional regulation, and two studies now describe a role for RBP1 Tyr1 phosphorylation in mammalian cells.