2019年10月31日 · We successfully identified many RGC subset markers and used hierarchical clustering analysis of the transcriptomes of these cells to reveal distinct populations of RGCs within the Pvalb +...

2018年7月17日 · Retinal ganglion cells (RGCs) convey the major output of information collected from the eye to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze...

神经上皮细胞主要由 Notch1 信号转导驱动，从而产生被称为放射状胶质细胞和雪旺细胞的相关细胞类型。放射状胶质细胞 (RGC) 保留了神经上皮特性，包括表达中间丝蛋白 Nestin 和 SRY 性别决定区 2 框 (Sox2)。

Retinal ganglion cells (RGC) transmit light and visual information to the brain via the long axons that form the optic nerve. Specifically, the human retina contains ~1.5 million RGCs, and they are located mainly in the ganglion cell layer and some of them in the inner nuclear layer [4].

2023年1月3日 · Here, we reviewed and analyzed the specificity of nine markers used to identify all or most RGCs, i.e., pan-RGC markers, in rats, mice, and macaques. The best markers in the three species in terms of specificity, proportion of RGCs labeled, and indicators of viability were BRN3A, expressed by vision-forming RGCs, and RBPMS, expressed by vision ...

2021年3月5日 · Using scRNA-seq on wild-type and mutant retinal cells, we reveal a transitional cell state of retinal progenitor cells (RPCs) co-marked by Atoh7 and other genes for different lineages and shared by...

In this article, Langer and colleagues present extensive characterization of RGC subtypes derived from human pluripotent stem cells, with multiple subtypes identified by subtype-specific molecular markers. Their results present a more detailed analysis of RGC diversity in human cells and yield the use of different markers to identify RGC subtypes.

In the present study, we developed a novel flow cytometry-based protocol to generate a homogeneous RGC population from murine retinae. We employed a highly stringent sort strategy coupled with qualitative PCR (qPCR) and intracellular staining with RGC-signature markers to verify the purity and homogeneity of the enriched population.

2014年4月15日 · There are few neurochemical markers that reliably identify retinal ganglion cells (RGCs), which are a heterogeneous population of cells that integrate and transmit the visual signal from the retina to the central visual nuclei. We have developed and characterized a new set of affinity-purified guine …

2022年4月13日 · Under the control of neuronal-specific promoters, the expression of fluorescent proteins, such as green fluorescent protein, can act as a cell-specific marker for RGC. Transgenic mice expressing fluorescent molecules observed by spectral-domain OCT and cSLO have made it possible to measure changes in RGC density and layer thickness over time ...