2022年1月4日 · RNA devices integrate cellular information sensing, processing and actuation of specific signals into defined functions and have yielded programmable biological systems and novel...

2015年8月10日 · We describe a framework for engineering RNA devices from preexisting aptamers that exhibit ligand-responsive ribozyme tertiary interactions.

2019年9月23日 · Here we report developing a quantitative, rapid and high-throughput mammalian cell-based RNA-Seq assay to efficiently engineer RNA devices. We identify new ribozyme-based RNA devices that...

We developed a general approach for assembling RNA devices that can execute higher-order cellular information processing operations from standard components. The engineered devices can function as logic gates (AND, NOR, NAND, or …

2024年2月1日 · Engineered RNA-based genetic controllers provide compact, tunable, post-transcriptional gene regulation. As RNA devices are generally small, these devices are portable to DNA and RNA viral vectors. RNA tools have recently expanded to allow reading and editing of endogenous RNAs for profiling and programming of transcriptional states.

2024年3月20日 · Here, we use the RNA origami method to develop an RNA robotic device, named the “Traptamer.” This device can sense two RNA key strands, function as a Boolean AND gate, and trigger the activation of the iSpinach fluorescent aptamer by releasing it from a mechanical trap.

2019年10月1日 · Biomolecule-responsive RNA devices are preferred components of mammalian synthetic gene circuits, because they enable cell-specific gene regulation with a low risk of insertional mutagenesis. In addition, because the output of one RNA device can be the input of another RNA device, synthetic biologists can construct layered gene circuits for ...

2019年9月23日 · Here we report developing a quantitative, rapid and high-throughput mammalian cell-based RNA-Seq assay to efficiently engineer RNA devices. We identify new ribozyme-based RNA devices that respond to theophylline, hypoxanthine, cyclic-di-GMP, and folinic acid from libraries of ~22,700 sequences in total.

2018年5月4日 · We developed a synthetic riboswitch that efficiently controls alternative splicing of a cassette exon in response to the small molecule ligand tetracycline. The riboswitch was designed to control the accessibility of the 3' splice site by placing the latter inside the closing stem of a conformationally controlled tetracycline aptamer.

Synthetic RNA devices sense drugs and metabolites to precisely and dynamically control gene expression inside cells, with the potential to transform medicine and biomanufacturing. The unique advantage of RNA devices lies in RNA's capacity to generate novel …