In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a great impact on RNA folding, will also be discussed.

Complementing the cellular results with the FTIR analysis of extracted RNA, ds-DNA, ss-cDNA and isolated nuclei, we verified that the spectral component centered at ∼1220 cm –1 is a good qualitative and semiquantitative marker

2024年10月16日 · This database encompasses a collection of FTIR spectra covering diverse nucleic acid molecules, including DNA, RNA, DNA/RNA hybrids and their various derivatives. NAIRDB covers details of the experimental conditions for FTIR measurements, literature links, primary sequence data, information about experimentally determined structures for related ...

The database contains an extensive collection of Fourier Transform Infrared (FTIR) spectra for a wide range of nucleic acid molecules, including DNA, RNA, and their derivatives. The NAIRDB database provides a systematic collection of FTIR data sets, with detailed descriptions of experimental conditions, including sample preparation, data ...

2005年8月26日 · We introduce time-resolved infrared spectroscopy as a powerful method to study the kinetics of RNA folding and unfolding transitions. A laser-induced temperature jump is used to initiate a perturbation in the RNA structure. A probe laser, tuned to a specific infrared absorption of the RNA, is then used to monitor the subsequent relaxation kinetics.

2024年10月16日 · 核酸红外数据库 （nairdb） 是一个全面的公共存储库，专门用于存档和免费分发特定于核酸的傅里叶变换红外 （ftir） 光谱数据。 该数据库包含一系列 FTIR 光谱，涵盖不同的核酸分子，包括 DNA、RNA、DNA/RNA 杂交体及其各种衍生物。

This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and interactions. Chapters cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance; FTIR, SAXS and SANS; and CryoEM.

2020年2月1日 · In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA.

2000年10月2日 · Fourier transform infrared (FTIR) spectra of RNA isolated from tumour brain (glioma) and DNA isolated from low-dose gamma-irradiated epididymis cells of rats from the Chernobyl accident zone have been investigated in the 700–4000 cm −1 region. The total absorbed radiation doses were equal to or less than 57 cGy.

2020年2月1日 · In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a …