2019年12月1日 · Extensive biochemical and structural studies of RNases H provided not only a comprehensive and complete picture of their mechanism but also revealed key basic …

RNases H are divided into two main classes (RNases H1 and H2 or type 1 and type 2 enzymes) with common structural features of the catalytic domain but different range of substrates for …

RNase H1 degrades the RNA portion of the R-loops returning the two DNA strands to double-stranded form (dsDNA). Overexpression of RNase H1 in different systems has helped to …

2024年8月29日 · RNase H1 functions as an endonuclease degrading the RNA moiety within RNA–DNA hybrids. Here the authors find that RNase H1 displays 3′-to-5′ exonuclease activity.

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. …

In this paper, we show that RNase H1 plays an important role in mtDNA transcription. Mutant RNASEH1 skin fibroblasts showed a significant decrease in some mitochondrial transcripts, …

2007年10月26日 · We report here crystal structures of human RNase H1 complexed with an RNA/DNA substrate. Unlike B. halodurans RNase H1, human RNase H1 has a basic …

R-loops are three-stranded structures that can pose threats to genome stability. RNase H1 precisely recognizes R-loops to drive their resolution within the genome, but the underlying …

2015年10月10日 · RNase H1 overexpression (black line) increased the degradation rate significantly to 24.6 fg/min, while reduction of RNase H1 (blue line) had the opposite effect, …

We report that RNase H1 is essential for processing of RNA primers to allow site-specific initiation of mtDNA replication. Without RNase H1, the RNA:DNA hybrids at the replication origins are …