We propose that the third hyper-reflective band in the porcine retina was generated by the extensive membranous profiles associated with the overlapping RPE apical cell processes and...

2016年6月9日 · We report a detailed protocol to collect, seed, culture and characterize RPE cells from mice. We describe a reproducible method that we previously developed to collect and culture murine RPE...

透射电子显微镜(transmission electron microscope，TEM)是检测细胞超微结构的常用工具，目前许多研究用TEM检测RPE细胞超微结构时是将体外培养的RPE细胞消化后观察沉淀的细胞 [6,7,8,9,10] ，但该方法会破坏RPE细胞原本的生长状态，不能真实地反映体外培养RPE细胞的超微 ...

TEM has been used extensively to visualize phagosomes within RPE. When using TEM, POS phagosomes inside the RPE can be unequivocally identified by size as well as their morphology, with distinct outer segment discs of POS readily apparent [2,14,17].

We propose that movement along this continuum toward a mesenchymal phenotype be defined as RPE Dysfunction. Potential mechanisms include impaired tight junctions, accumulation of misfolded proteins and dysregulation of several key pathways and molecules, such as TGF-β pathway, Wnt pathway, nicotinamide, microRNA 204/211 and extracellular vesicles.

Nonpolarized RPE cells were treated with 150 μM tBH or 10 μg HN or 10 μg HN + 150 μM tBH for 24 hours and samples were analyzed for mitochondrial biogenesis by DNA copy number, TEM for mitochondrial number, and Western blot analysis for mtTFA.

RPE细胞的收集需要约3小时，并且培养物可在1周内模拟体内RPE细胞的特征。该协议还描述了通过透射和扫描电子显微镜（分别为TEM和SEM），振动切片和平坦支架的免疫染色以及跨上皮电阻测量来表征1-2周内Transwell细胞的方法。

Age-related Macular Degeneration (AMD), a blinding eye disease, is characterized by pathological protein- and lipid-rich drusen deposits underneath the retinal pigment epithelium (RPE)...

TEM images of RPE-retina interphase of embryonic (E14.5) and adult (P30) mice (see details in the Materials and Methods section). Images in the right column are the magnified versions of the...

结果：Mfp2 -/- RPE 中的第一个病理征兆是通过 Perilipin-2 染色观察到的 3w 时的脂质积累，而在 2w 时不存在，这表明过氧化物酶体 β-氧化对于消化来自摄入 POS 的脂质很重要，富含VLC-PUFA。