In human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs)–derived RPE cells, the expression of several AQPs was determined by quantitative real-time PCR and the localization of AQP1 was assessed with confocal microscopy. The functionality of AQP water channels was determined by cell volume assay in hESC-derived RPE cells. Results.:

Results: AQP1 protein was detected in human RPE in situ and in cultures of human adult and fetal RPE cells. In functional assays, AQP1 facilitated water movement across RPE monolayers in an expression-dependent manner in two complementary model systems.

2012年4月26日 · The expression of aquaporin (AQP) water channels may influence the development of retinal edema. We investigated the transcriptional regulation of AQP3 in cultured human retinal pigment epithelial (RPE) cells. As shown by RT-PCR and immunocytochemistry, cultured RPE cells express AQP3 mRNA and protein.

2009年9月1日 · 水通道蛋白 (aqp) 是承担此功能的良好候选者。 在这项工作中，我们研究了渗透压对 RPE 细胞中 AQP 表达的影响。 方法 ARPE-19细胞在不同的高渗条件下生长。

2013年5月1日 · The localization of AQP1 on the apical plasma membrane in mature RPE cells derived from both hESC and hiPSC suggests its functionality. These data propose that hESC- and hiPSC-derived RPE cells, grown and differentiated under serum-free conditions, resemble their native counterpart in the human eye.

2020年12月30日 · The present study was performed to investigate in cultured human RPE cells the subcellular localization of AQP8 and the cellular mechanisms of hyperosmotic AQP8 expression, and to investigate whether knockdown of AQP8 has effects on three important physiologic properties of RPE cells implicated in retinal diseases in situ: cell proliferation ...

2024年2月6日，瑞典隆德大学医学院黄朋/Karin Lindkvist教授共通讯在国际著名期刊PNAS上发表了题为“Molecular basis for human aquaporin inhibition”的研究，这是该团队继2023年2月在Nature Communications上发表”Cryo-EM structure suppports a role of AQP7 as a junction protein”（详 …

Blood-retinal barrier (BRB) breakdown and retinal edema are major complications of autoimmune uveitis and could be related to deregulation of aquaporin (AQP) expression. We have therefore evaluated the expression of AQP1 and AQP4 on BRB cells during ...

2015年4月9日 · RPE cells transfected with NFAT5 siRNA displayed a significantly (p<0.05) reduced upregulation of AQP5 (Figure 10F) and VEGF expression (Figure 10G), and a significantly (p<0.05) reduced secretion of VEGF (Figure 10H) under hyperosmotic conditions compared to non-transfected cells.

水通道蛋白家族（AQPs）是水分子跨膜转运主要分子基础，参与水的双向转运和细胞内外水平衡，对维持机体正常生理功能具有重要作用。 20世纪80年代，在红细胞膜上发现第一个AQPs，后被正式命名为AQP1。 随着分子生物学发展，在哺乳动物中陆续发现不同的AQPs，已鉴定至少200种。 一、关于AQPs的调节机制. AQPs是一组对水有高度选择性的膜通道蛋白，它们在哺乳动物的组织和器官中广泛分布，至今已发现13种（AQP0-AQP12）。 AQPs在循环、泌尿、神经、运 …