pKDsgRNA-rpsL - Addgene
Plasmid pKDsgRNA-rpsL from Dr. Michael Lynch's lab contains the insert rpsL gRNA and is published in ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174. This plasmid is …
T7 promoter + rpsL reporter plasmid - Addgene
Plasmid T7 promoter + rpsL reporter plasmid from Dr. Matthew D. Shoulders's lab contains the inserts rpsL and TcR and is published in J Am Chem Soc. 2018 Sep 19;140 (37):11560 …
pCRISPR::rpsL - Addgene
Plasmid pCRISPR::rpsL from Dr. Luciano Marraffini's lab contains the insert CRISPR::rpsL and is published in Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. This plasmid is available …
Creating custom synthetic genomes in Escherichia coli with …
2016年10月24日 · We previously developed REXER (Replicon EXcision Enhanced Recombination); this method enables the replacement of >100 kb of the Escherichia coli …
自杀质粒介导的谷棒基因编辑新方法_-rpsL - 搜狐
2019年8月8日 · 本研究报道了一种新的反筛标签-rpsL（编码核糖体小亚基的S12蛋白），用其作为反筛标签进行二轮筛选，表型阳性率大大提高。 具体为将大肠来源2个关键基因引入谷棒菌 …
Sensing Cytosolic RpsL by Macrophages Induces Lysosomal Cell
2015年3月4日 · Our results identified RpsL as a regulator in the interactions between bacteria such as L. pneumophila and primary mouse macrophages by triggering unique cellular …
Anti-rpsL Antibodies | Invitrogen - 赛默飞世尔科技公司
Antibodies that detect rpsL can be used in several scientific applications, including ELISA and Western Blot. This Rabbit polyclonal antibody targets rpsL, and has been validated in …
pP10-rpsLneo 载体图谱和序列 - 纽普生物
我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。 所有的产品都严格仅供科学研究使用，不能 …
Genetically engineered rpsL merodiploidy impacts secondary …
2021年3月17日 · Introduction of mutant rpsL alleles in the rpsLWT background can be used to circumvent these obstacles. Here we take the latter approach and report the generation and …
Simple generation of site-directed point mutations in the
2008年4月24日 · We used rpsL counter-selection in combination with Red ® /ET ® Recombination to introduce a single point mutation into the kdpA gene locus of the MG1655 …