We developed a high-throughput, primary cell-based antiviral RSV assay to enable identification of small molecules using cytopathic effect (CPE) as a phenotypic end point.

2011年1月27日 · Consistent with the CPE data, most cells were positive for RSV F protein by 72 hpi with RSV A2, indicating extensive infection and efficient virus propagation. In contrast, but also consistent with the CPE data, few cells were infected with the RSV clinical isolates, with BT2a and BT4a the most and least infectious, respectively.

2014年10月3日 · Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome....

2021年5月18日 · A high-throughput screen of a Roche internal chemical library based on inhibition of the respiratory syncytial virus (RSV)-induced cytopathic effect (CPE) on HEp-2 cells was performed to identify RSV inhibitors. Over 2,000 hits were identified and confirmed to be efficacious against RSV infection …

2015年7月1日 · We developed a high-throughput, primary cell-based antiviral RSV assay to enable identification of small molecules using cytopathic effect (CPE) as a phenotypic end point. To provide increased biological relevance, we developed our assay with primary human small airway epithelial cells (SAECs), which originate from known sites of RSV infection ...

2024年12月24日 · Methods: The antiviral effect of 320 compounds on RSV in HEp-2 cells was tested using a Cytopathic effect (CPE) inhibition assay. The antiviral effect of fumarprotocetraric acid (FUM) and geraniin (GE) were confirmed by Real-time reverse transcription quantitative PCR (Real-time RT-PCR), plaque reduction test, immunofluorescence assay, and ...

Since the direct observation of viral particles requires the use of electron microscopy techniques, detection of the CPE is considered an alternative way to recognize a viral infection with conventional equipment.

When combined with follow-up live virus CPE assays, we identified three novel diversity-oriented synthesis-derived scaffolds, which bear no resemblance to known RSV inhibitors including YM-53403, that inhibit the replication of RSV, but not influenza or HSV.

Respiratory syncytial virus (RSV) is the most important cause of lower respiratory tract disease in infants and children. To study RSV replication, we have developed an in vitro model of human nasopharyngeal mucosa, human airway epithelium (HAE).

RSV A2 induces more cytopathic effects (CPE) than the 3 clinical isolates. PBECs from 5 individual donors were infected with RSV A2, BT2a, BT3a and BT4a (MOI of 0.1) during 2 h and washed with PBS. Seventy two hpi, cells were examined by phase contrast microscopy. Micrographs of a representative field for each RSV strain are presented.