2018年1月24日 · Formation of inter-organelle contacts between mitochondria and lysosomes, regulated by lysosomal RAB7 GTP hydrolysis, allows for bidirectional regulation of mitochondrial and lysosomal dynamics.

2021年8月23日 · rab7的活性和动力学——包括它在自噬囊泡上的活性gtp结合状态和非活性gdp结合形式之间的转换——对于自噬通量的进展至关重要。 RAB7活性受到称为“Armus”（也称为 TBC1D2A）的GTP酶激活蛋白(GAP) 的负调控，其靶向作用是通过与自噬液泡上的LC3和VPS34在内吞体上 ...

溶酶体同样受到 rab7 gtpase 的持续动态调节，在 gtp 水解后，它从活跃的 gtp 结合状态循环到非活性的 gdp 结合状态。 在这里，我们使用电子显微镜、结构化照明显微镜和高空间和时间分辨率共聚焦活细胞成像确定了线粒体 - 溶酶体膜接触位点的形成和调节。

Rab小GTP酶（小G蛋白）是单体GTP结合蛋白，大约含有200个氨基酸。 ... 近年的研究表明，Rab4、Rab5、Rab7、Rab9、Rab10、Rab11和 Rab35与内吞运输有关。这些Rab位于不同内吞体或同一内吞体的不同区域，独立或协同调节内吞运输的不同步骤。

机制上，该研究利用磷酸化蛋白质组学，筛选并验证了Rab7a在S72位点的磷酸化阻碍了内毒素血症中EGFR从早期到晚期内吞体的转化，Rab7a磷酸化使其GTP酶失活，这阻断了膜受体-溶酶体降解途径导致质膜EGFR的高表达。

Both mutants exhibited decreased RILP binding (Fig. 6d and e), and a separate assay, measuring Rab7 protein binding to GTP-bound beads and thus activity per se , showed that only the CAAX mutant of Rab7 was impaired in activity . These data demonstrate that membrane association of Rab7 is required for its activation during lipophagy and that ...

2023年1月31日 · 背景介绍：Rab7属于小GTPases的Rab家族。Rab7位于晚期核内体、溶酶体、细胞质囊泡、吞噬体和黑素小体。它参与了晚期的内吞转运，并有助于吞噬体的成熟。 品牌: 美国 NewEast Biosciencesa供应商：武汉费斯德生物科…

2022年7月16日 · Rab7 is a GTPase that controls late endosome and lysosome trafficking. Recent studies have demonstrated that Rab7 is ubiquitinated, a post-translational modification mediated by an enzymatic cascade. ... Characterization of its ubiquitination reveals that Rab7 must be in its GTP-bound active form for membrane anchoring and, thus, accessible for ...

2018年2月15日 · Lysosomes similarly undergo constant dynamic regulation by the RAB7 GTPase, which cycles from an active GTP-bound state into an inactive GDP-bound state upon GTP hydrolysis. Here we have identified the formation and regulation of mitochondria-lysosome membrane contact sites using electron microscopy, structured illumination microscopy and high ...

Small GTPases of the Rab subfamily localise to the cytoplasmic face of distinct membrane compartments and act as regulators of intracellular membrane traffic and transport vesicle formation. Among them, Rab7 controls the delivery of internalised material into degradative compartments and the acquisition of lysosomal hydrolases. This activity shapes the endocytic pathway of every cell type. In ...