2020年11月3日 · We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8 -deficient cells lack Rab7...

2020年11月3日 · We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation and show severe defects in late endosome morphology and endosomal LDL trafficking, resulting in cellular cholesterol deficiency.

This review will discuss the GEF and GAPs that control Rab7 nucleotide binding, and thus regulate Rab7’s activity in endolysosomal trafficking and autophagy. It will also consider how bacterial pathogens manipulate Rab7 nucleotide binding to support intracellular invasion and immune evasion.

In metazoans, Rab7 controls endosomal maturation, autophagy, and lysosomal function. It is activated by the guanine nucleotide exchange factor (GEF) complex Mon1–Ccz1–Bulli (MCBulli) of the tri-longin domain (TLD) family.

2014年12月2日 · In this study, we report that RILP, a downstream effector of Rab7, interacts with HOPS complex and recruits HOPS subunits to the late endosomal compartment. Structurally, the amino-terminal...

2022年2月1日 · The best-studied TLD Rab-GEF is MC1, which activates Rab7 (Ypt7 in yeast) in endosomal maturation and autophagy. In yeast, it has been demonstrated that MC1-dependent recruitment of Ypt7 to both late endosomes/multivesicular bodies and autophagosomes is required for the fusion of these organelles with the vacuole and degradation of the ...

2023年5月24日 · 在所有真核生物中，Mon1-Ccz1复合物一方面作为RAB7的GEF（鸟苷酸交换因子），可以促进RAB7从结合GDP的静息态向结合GTP的活化态转化；同时，Mon1-Ccz1作为RAB5的效应因子（effector），可以促进内吞体上RAB5-RAB7的转化，以此调控内吞体-溶酶体的成熟过程【3】。

2020年7月9日 · Recruitment of Rab7 to endosomes requires the Mon1-Ccz1 guanine-nucleotide-exchange factor (GEF). Here, we show that full function of the Drosophila Mon1-Ccz1 complex requires a third stoichiometric subunit, termed Bulli (encoded by CG8270). Bulli localises to Rab7-positive endosomes, in agreement with its function in the GEF complex.

2019年8月1日 · Rab GTPases and their regulatory proteins play a crucial role in vesicle-mediated membrane trafficking. During vesicle membrane tethering Rab GTPases are activated by GEFs (guanine nucleotide exchange factors) and then inactivated …

2018年7月16日 · Rab GTPases are key factors that determine organelle identity throughout the endomembrane system. Endosomes change identity by replacing Rab5 with Rab7, which requires the specific and tightly regulated recruitment of the Rab7 guanine nucleotide exchange factor (GEF) to the endosome membrane.