2017年11月20日 · RAB7 is known to be a key organizer/regulator of the late endosomal/lysosomal network that associates with various effectors to orchestrate diverse functions such as lysosomal biogenesis, late endosome–lysosome fusion, autophagosome maturation, and cargo transport within the late endocytic network (Guerra & Bucci, 2016).

2018年4月6日 · In this study, we investigated the function of mammalian Rab7 in greater detail by using Rab7-knockout (KO) cells and found evidence that Rab7 is dispensable for autophagosome–lysosome fusion in cultured mammalian cells. Loss of Rab7 resulted in autolysosome accumulation, but not autophagosome accumulation, under fed conditions alone.

2024年11月19日 · Here we show that the small GTPase Rab7a strongly enhances the activity of TPC2 and that effects of TPC2 on melanoma hallmarks, in vitro and in vivo strongly depend on the presence of Rab7a, which...

Rab7基因，也称为RAB7A，是Rab GTP酶家族成员之一，广泛存在于真核细胞中，参与调控内吞作用、自噬作用和细胞内囊泡运输等生物学过程。 Rab7在细胞内囊泡运输中发挥着关键作用，它能够识别特定的囊泡，并招募相应的运输蛋白，从而实现囊泡的定向运输和融合。 此外，Rab7还参与自噬作用，通过调节自噬泡的形成和与溶酶体的融合，促进细胞内受损或衰老细胞器的清除和降解。 研究表明，Rab7基因在多种疾病中发挥重要作用，包括动脉粥样硬化、糖尿病心肌病 …

2024年8月5日 · Rab7 (serine 72) is a substrate of TBK1 at lysosomes. Rab7 suppresses mTORC1 activity and this is relieved by TBK1-dependent phosphorylation of Rab7 on serine 72. The ALS-FTD associated E696K TBK1 mutant accumulates at lysosomes resulting in increased Rab7 phosphorylation and mTORC1 activity.

敲除Rab7基因的exon 3 区域，获得Rab7基因敲除小鼠模型。 *使用本品系发表的文献需注明: Rab7-KO mice (Cat. NO. NM-KO-190355) were purchased from Shanghai Model Organisms Center, Inc.. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小 …

2018年11月8日 · However, a recent analysis of Rab7 knockout (KO) mammalian cultured cells generated by using the CRISPR/Cas9 system unexpectedly showed that loss of Rab7 phenotypes in mammals is clearly different from the loss of phenotype observed in yeast and fruit flies, and that mammalian Rab7 is involved in autolysosome maturation under nutrient-rich ...

2018年4月6日 · We found that knockout (KO) of Rab7 (herein referring to the Rab7a isoform) caused an accumulation of a massive number of LC3-positive autolysosomes under nutrient-rich conditions, indicating that Rab7 is dispensable for autophagosome-lysosome fusion.

2018年11月19日 · Rab7 (or Ypt7 in yeast) is one of the well-characterized members of the Rab family small GTPases, which serve as master regulators of membrane trafficking in eukaryotes. It localizes to late endosomes and lysosomes and has multiple functions in the autophagic pathway as well as in the endocytic path …

2020年4月3日 · Three independent papers showed that Rab2 mediates autophagosome-lysosome fusion in flies, while the roles of Rab2A (and Rab2B) are not that clear in mammalian cells. In mammals, autolysosomes can form in Rab7 KO cells unlike in invertebrates or fungi, indicating increasing gene redundancy.