2017年3月1日 · Raman probes, including SERS tags, bioorthogonal Raman tags, and RR tags, have emerged as optical probes beyond fluorescence imaging and label-free Raman imaging. Every kind of Raman probe has its advantages and disadvantages. With ultra-high sensitivity, SERS tags are suitable for detecting low-concentration molecules.

2019年8月29日 · In this work, we report ultrabright gap-enhanced Raman tags (GERTs) with strong electromagnetic hot spots from interior sub-nanometer gaps and external petal-like shell structures, larger...

We report the design and synthesis of orthogonal gap-enhanced Raman tags (O-GERTs) by embedding alkyne and deuterium-based reporters in the interior metallic nanogaps of core–shell nanoparticles and explore their signal orthogonality as optical probes against different backgrounds from common substrates and media (e.g., glass and polymer) to ...

Mapping the Raman signal intensity of alkyne tags observed in the cell silent region is called alkyne tag Raman imaging (ATRI). The alkyne moiety can be used as an exogenous tag because it has a unique Raman signature of its stretching vibration that does not overlap with any signals of biological molecules.

As a newly emerging category of tags for molecular imaging in live cells, the Raman label attracts much attention because of the rich information obtained from targeted and untargeted molecules by detecting molecular vibrations.

Gap-enhanced Raman tags (GERTs) are emerging probes of surface-enhanced Raman scattering (SERS) spectroscopy that have found promising analytical, bioimaging, and theranostic applications. Because of their internal location, Raman reporter molecules ...

2024年4月4日 · Spectroscopic studies increasingly employ Raman tags exhibiting a signal in the cell – silent region of the Raman spectrum (1800–2800 cm −1), where bands arising from biological molecules are inherently absent. Raman tags bearing functional groups which contain a triple bond, such as alkyne and nitrile or a carbon–deuterium bond, have a ...

2020年5月31日 · Chemical contrast in cellular imaging can be achieved by bioorthogonal labeling of molecules with small Raman tags, such as C D or C C groups giving rise to signals in the silent wavenumber region of a cellular Raman spectrum. Typical regions of generated signals are shown on the right side

2017年3月1日 · Raman probes, including SERS tags, bioorthogonal Raman tags, and RR tags, have emerged as optical probes beyond fluorescence imaging and label-free Raman imaging. Every kind of Raman probe has its advantages and disadvantages. With ultra-high sensitivity, SERS tags are suitable for detecting low-concentration molecules.

2024年7月3日 · In this work, we report a novel petal gap-enhanced Raman tag (GERT) consisting of a bipyramid core and a nitrothiophenol (NTP) spacer to support the growth of hundreds of small petals and compare its SERS emission and localization to a traditional bipyramid aggregate.