008477 STOCK Tg (RARE-Hspa1b/lacZ)12Jrt/J These transgenic mice express beta-galactosidase (<em>lacZ</em>) gene under the control of the retinoic acid responsive element (RARE). Retinoic acid signaling is indicated by beta-galactosidase activity. This mutant mouse strain may be useful in tracking retinoic acid signaling pattern.

LacZ基因是细胞内重要的 reporter基因，其产物是β-半乳糖苷酶，其底物为半乳糖苷，当把此底物标记上荧光基团后，即成为底物荧光探针。 LacZ基因广泛用于基因表达调控研究中的一种基因。 例如，基因克隆中常用的质粒载体PUC 19及噬菌体载体M13系列均带有LacZ基因。

2011年1月11日 · The RARE-lacZ RA-reporter transgene, which places lacZ (encoding β-galactosidase) under the control of a RARE, was used for in situ detection of RA activity in embryonic...

2019年7月30日 · Using a liquid chromatography/mass spectrometry-based method to quantify trace amounts of RA in embryonic tissue requires expensive specialist facilities. Here, we describe the use of a RA response element (RARE)-lacZ reporter cell-based method, which is simple and cost effective, to measure RA levels in small pieces of tissue from the embryo.

2023年9月21日 · R26R鼠 （ROSA-LSL-LacZ）是通过pROSA26-1载体构建的一种reporter鼠，插入了一段LSL序列和LacZ序列，如图5A。 这种鼠正常情况下并不表达LacZ，用LacZ的底物 X-Gal 对组织切片染色并不能显色；而被Cre重组后，由于LSL序列被敲除，LacZ表达，在小鼠的组织切片中用X-Gal染色就会发生蓝色的显色反应，如图5B。 当然啦，由于LacZ鼠只能通过X-Gal显色，所以仅适用于体外检测. mT/mG（ROSA-pCA-loxp-mT-pA-loxp-mG-pA）鼠的设计就更精妙 …

Originally used to map embryonic retinoic acid (RA) signaling, RARE-LacZ mice possess multiple LacZ genes controlled by retinoic acid response elements (RAREs). We crossed RARE-LacZ mice with AD mouse models to determine their suitability for quantitative studies into the effects of VA on dentate gyrus (DG) RA signaling and learning in AD.

背景：细菌的乳糖操纵子（lac）包含一个叫lacZ的基因，它编码的蛋白是β - 半乳糖苷酶。 乳糖操纵子可以被乳糖或者乳糖类似物 IPTG 给激活，启动下游基因的表达。

2016年9月6日 · As a reporter system, the F9 RARE-LacZ cell line can be used in a variety of ways, from simple detection of RA to quantitative measurements of RA levels in tissue explants. Here we describe the quantitative determination of relative RA levels generated in embryos and neurosphere cultures using the F9 RARE-LacZ reporter cell line.

Raldh2−/+ mice were mated to RARE-lacZ mice to generate a strain of Raldh2−/+ mice carrying RARE-lacZ, identified by Southern blot analysis. Embryos were stained 6 hours for lacZ expression (β-galactosidase activity) using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as substrate (Hogan et al., 1994).

2020年10月7日 · Retinoic acid (RA) activates RA receptors (RAR), resulting in RA response element (RARE)-dependent gene expression in renal collecting duct (CD). Emerging evidence supports a...