Feb 3, 2021 · RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA.

In Arabidopsis, genetic evidence demonstrates that RNA-dependent RNA polymerase 6 (RDR6) plays a fundamental role in at least four RNA silencing pathways whose functions range from defense against transgenes or viruses to endogene regulation in development and in …

Apr 14, 2018 · Plant small RNAs (sRNAs) modulate key physiological mechanisms through post-transcriptional and transcriptional silencing of gene expression. Small RNAs fall into two major categories: those are reliant on RNA-dependent RNA polymerases (RDR s) for biogenesis and those that are not.

May 8, 2014 · Recent research has uncovered variations on the canonical RdDM pathway, including the involvement of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) and NEEDED FOR RDR2-INDEPENDENT DNA METHYLATION...

Based on these results, we propose that NbRDR6 participates in the antiviral RNA silencing pathway that is stimulated by rising temperatures but suppressed by virus-encoded silencing suppressors. The relative strengths of these two factors, along with other plant defense components, critically influence the outcome of virus infections.

Mar 20, 2017 · Here, we show that RDR6 itself selects aberrant poly (A)-less mRNAs over canonical polyadenylated mRNAs as templates at the initiation step of complementary strand synthesis. This mechanism can...

Sep 18, 2024 · RDR6-RdDM functions to recognize active Polymerase II-derived Transposable element (TE) mRNA transcripts to both trigger and correctively reestablish TE methylation and epigenetic silencing. RDR6 is required for efficient hpRNA-induced RNA silencing in plants.

Here we report that OsRDR6, the rice (Oryza sativa L.) homolog of Arabidopsis RDR6, acts in the biogenesis of various types and sizes of small RNAs. We isolated a rice osrdr6-1 mutant, which was temperature sensitive and showed spikelet defects.

Aug 13, 2024 · RDR6 mainly converts Pol II-produced ssRNA into dsRNA. This dsRNA is further cleaved by DCL2 or DCL4 to 22-nt or 21-nt siRNAs (Fig. 1c), which are ultimately loaded onto AGO1 (Borges & Martienssen, 2015). Unlike RDR2, which directly interacts with Pol IV, RDR6 does not associate with Pol II.

RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA.