Structure of replicating SARS-CoV-2 polymerase - Nature
2020年5月21日 · Here we present a cryo-electron microscopy structure of the SARS-CoV-2 RdRp in an active form that mimics the replicating enzyme. The structure comprises the viral proteins non-structural protein...
Architecture of a SARS-CoV-2 mini replication and transcription complex
2020年11月18日 · Here we determine the atomic structure of a SARS-CoV-2 mini RTC, assembled by viral RNA-dependent RNA polymerase (RdRp, nsp12) with a template-primer RNA, nsp7 and nsp8, and two helicase...
Protein-protein interaction of RdRp with its co-factor NSP8 and …
2022年6月6日 · In this study we explored the molecular mechanism of RdRp (Non-Structural Protein, NSP12) interaction with its co-factors NSP7 and NSP8 which is the main toolbox for RNA replication and transcription of SARS-CoV-2 and SARS-CoV.
Two conserved oligomer interfaces of NSP7 and NSP8 underpin …
Replication of the ∼30 kb-long coronavirus genome is mediated by a complex of non-structural proteins (NSP), in which NSP7 and NSP8 play a critical role in regulating the RNA-dependent RNA polymerase (RdRP) activity of NSP12. The assembly of NSP7, NSP8 and NSP12 proteins is highly dynamic in solution, yet the underlying mechanism remains elusive.
Structural Basis for RNA Replication by the SARS-CoV-2 Polymerase
2020年7月23日 · Cryo-EM structures of the SARS-CoV-2 RNA polymerase in complexes with RNA, before and after RNA translocation, reveals structural rearrangements that the RNA-dependent RNA polymerase (RdRp) nsp12 and its co-factors (nsp7 and nsp8) undergo to accommodate nucleic acid binding.
The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric …
Uniquely among RNA viruses, replication of the ∼30-kb SARS-coronavirus genome is believed to involve two RNA-dependent RNA polymerase (RdRp) activities. The first is primer-dependent and associated with the 106-kDa non-structural protein 12 (nsp12), whereas the second is catalysed by the 22-kDa nsp8.
Multiscale characterization reveals oligomerization dependent …
2022年9月7日 · At least two RNA-dependent RNA polymerases (RdRp) are encoded by the coronavirus genome, including primer-dependent RdRp nsp12 4 and primer-independent RdRp nsp8 5, 6. Recent structural...
Transient and stabilized complexes of Nsp7, Nsp8, and Nsp12 in …
2021年8月3日 · We have conducted a systematic structural investigation of three components that make up the RTC: Nsp7, Nsp8, and Nsp12 (also known as RNA-dependent RNA polymerase). We have solved high-resolution crystal structures of the Nsp7/8 complex, providing insight into the interaction between the proteins.
Mutations in SARS‐CoV‐2 nsp7 and nsp8 proteins and their …
2021年3月14日 · RdRp forms a complex with nonstructural proteins nsp7 and nsp8 to form the minimal replication/transcription machinery required for genome replication. As mutations in RdRp may affect formation of the RdRp–nsp7–nsp8 supercomplex, we analyzed viral genomes to identify mutations in nsp7 and nsp8 protein sequences.
NSP7 和 NSP8 的两个保守寡聚体界面支持 SARS-CoV-2 RdRP 的 …
约 30 kb 长的冠状病毒基因组的复制由非结构蛋白 (NSP) 复合物介导，其中 NSP7 和 NSP8 在调节 NSP12 的 RNA 依赖性 RNA 聚合酶 (RdRP) 活性中起关键作用。 NSP7、NSP8 和 NSP12 蛋白的组装在溶液中是高度动态的，但其潜在机制仍然难以捉摸。