Rhamnolipids are a class of glycolipid produced by Pseudomonas aeruginosa, amongst other organisms, frequently cited as bacterial surfactants. [1][2][3] They have a glycosyl head group, in this case a rhamnose moiety, and a 3- (hydroxyalkanoyloxy)alkanoic acid (HAA) fatty acid tail, such as 3-hydroxydecanoic acid. [4][5]

2023年4月13日 · Rhamnosyltransferase 1 chain A (RhlA) catalyzes the formation of β-3- (3-hydroxyalkanoyloxy)alkanoic acids (HAAs) to constitute the RL lipid moiety, and the molecular structure of this moiety exerts major impacts on the physiochemical and biological properties of corresponding RLs.

2023年4月18日 · 鼠李糖脂（Rhamnolipid，RL）是一种最具应用潜力的生物表面活性剂。 RhlA酶是影响RL产物结构的关键因素，但其序列-结构-功能关系尚不清楚，且催化机制存在争议。

2021年10月6日 · Our data indicate that rhamnolipids do not have such a crucial role in achieving surface colonization of non-agar plates, suggesting a strong dependence on the physical properties of the tested surface. The use of alternative gelling agent provides new means to reveal unknown features of bacterial surface behaviour.

铜绿假单胞菌分泌一种鼠李糖脂 (RL) 表面活性剂，其在疏水性营养吸收、蜂群运动和发病机制中起作用。 我们表明，RhlA 通过与 II 型脂肪酸合酶 (FASII) 循环的酶竞争β-羟酰基-酰基载体蛋白 (ACP) 途径中间体，为 RL 生物合成提供酰基部分。 纯化的 RhlA 从两分子 β-羟基癸酰基-ACP 形成一分子 β-羟基癸酰基-β-羟基癸酸酯，并且是生成 RL 脂质成分所需的唯一酶。 RL 中的酰基主要是 β-羟基癸酰基，在体外，RhlA 对 10 碳底物具有更大的亲和力，说明 RhlA 作为分子标尺从 …

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Pseudomonas aeruginosa produces the biosurfactant rhamnolipid, which has several potential biotechnological applications. The synthesis of this surfactant is catalyzed by rhamnosyltransferase 1, composed of the proteins RhlA and RhlB. Here we report that RhlA plays a role not only in surfactant synt …