2003年7月1日 · We show that the ERK-MAPK pathway downstream of oncogenic Ras can regulate the activity of the small GTPases Rac and RhoA and thereby contribute to tumor cell motility and invasion. ERK-MAPK signaling to RhoA is via transcriptional upregulation of the Fra-1 transcription factor and to Rac via transcriptional upregulation of the urokinase ...

2016年1月27日 · Here, we provide evidence that RhoA is phosphorylated by ERK on 88 S and 100 T in response to EGF stimulation. We show that ERK interacts with RhoA and that this interaction is dependent on the ERK docking site (D-site) at the C-terminus of RhoA. EGF stimulation enhanced the activation of the endogenous RhoA.

2016年1月27日 · Here, we provide evidence that RhoA is phosphorylated by ERK on 88S and 100T in response to EGF stimulation. We show that ERK interacts with RhoA and that this interaction is dependent on the ERK docking site (D-site) at the C-terminus of RhoA. EGF stimulation enhanced the activation of the endogenous RhoA.

Y27632 是 ROCK1/2 的特异性强效抑制剂，ROCK1/2 是 RhoA 和 RhoC 的主要效应因子，作用于参与肌动蛋白聚合的下游通路。 它没有改变静态条件下 CTGF 的基础表达，但抑制了应激诱导的 CTGF 上调，无论是在 mRNA（图2 E）或蛋白质水平（图2 F-G）。 由于 RhoA 在这个过程中是可有可无的（图2 B），这进一步证实了 RhoC 参与了 VIC 对机械信号的响应。 图2 RhoC 通过 ROCK1/2 调节机械应力诱导的 CTGF 表达。 MEK/Erk 通路 被机械应力激活，独立于 RhoC 和 …

2013年7月4日 · RhoA, regulating Rho-associated coiled-coil-forming kinase 1 (ROCK1), modulates multiple cell functions, including malignant transformation, metastasis and cell death. Therefore, RhoA/ROCK1...

2003年4月11日 · Inhibition of either ERK or RhoA signals prevents chemotaxis, indicating that both pathways contribute to the establishment of cell polarity and migration. Together, these findings demonstrate that ERK and RhoA are distinct pathways that control pseudopodia extension and retraction, respectively, through differential modulation of MLC ...

2008年12月11日 · We examined whether RhoA can substantially affect ERK activity via this MEKK1-mediated crosstalk between RhoA and EGFR-ERK pathway. By extending the published EGFR-ERK simulation models represented by ordinary differential equations, we developed a simulation model that includes this crosstalk, which was validated with a number of experimental ...

2018年10月29日 · Specifically, RhoA and ROCK repress ERK kinase activity, which promotes JunB and Nfix expression. Notably, the role of ERK in the activation of Nfix is conserved postnatally in satellite cells, which represent the canonical myogenic stem cells of adult muscle.

2022年6月2日 · Here we report that in TGFβ-induced EMT, ERK-mediated Smurf1 phosphorylation is a prerequisite step for RhoA degradation and the consequent mesenchymal state achievement. Upon TGFβ treatment, activated ERK phosphorylates Thr223 of Smurf1, a member of HECT family E3 ligase, to promote Smurf1-mediated polyubiquitination and degradation of RhoA ...

We studied the relationship between RhoA and ERK-MAPK signaling to determine their roles in the regulation of osteogenesis by ECM compliance. Inhibition of RhoA and ROCK in MC3T3-E1 pre-osteoblasts cultured on substrates of varying compliance reduced ERK activity, whereas constitutively active RhoA enhanced it.