2016年1月27日 · Using a small chemical inhibitor of PAK, we demonstrate that cellular RhoA and Rac1 activation levels respond in a history-dependent, bistable manner to PAK inhibition. …

2024年2月19日 · Rho GTPases 是一类重要的细胞信号传导蛋白，在细胞内起着关键的调控作用。 它们是小的 GTP酶，属于 Ras超家族，包括RhoA、RhoB、RhoC、Rac1、Rac2、Cdc42等 …

Here we focus on our current understanding of the way in which different Rho-associated serine/threonine kinases, denoted PAK (p21-activated kinase), MLK (mixed-lineage kinase), …

Rac1 has been shown to reduce RhoA activity through multiple mechanisms, some of which are dependent on the downstream effector kinase PAK. 5 PAK has been reported to …

RhoA binds to Rhophilin and regulates the actin cytoskeleton. RhoA also interacts with a Rho target protein, Rhotekin through the RBD motif. RhoA-dependent signaling is recognized as an …

Using predictive mathematical modeling, mass spectrometry-based quantitation of network components, and experi-mental validation in MDA-MB-231 mesenchymal breast cancer cells, …

2018年7月4日 · Recently, it was shown that Rac1 and RhoA activity and downstream signaling respond in a bistable manner to perturbations of this network. Keywords: PAK inhibition; Rac1; …

Constitutively active RhoA Rac1-GTP (a) A constitutively active, EGFP tagged RhoA V14 mutant was transfected into MDA-MB-231 cells. Rac1-GTP was precipitated with GST-PAK-CRIB …

2010年1月12日 · 没有观察到Rac1，Cdc42或p21-PAK的主要免疫组化变化，因此鉴定出RhoGTPase中的RhoA是AD中可能的治疗靶标。 提示RhoA的亚细胞靶向改变。 在AD …

These data provide a novel mechanism for the control of RhoA activity by Rac1 through the PAK-dependent phosphorylation of NET1 to reduce its activity as a guanine nucleotide exchange …