2015年12月1日 · We were able to confirm that there was no unspecific precipitation of cellular RNA or G3BP1 by including normal IgG antibodies as RIP negative control. Additionally, the absence other mRNAs, like β-actin or Trim5α, allowed us to rule out unspecific binding of G3BP1 to cellular RNAs.

2019年12月11日 · Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor and is involved in the innate immune response against RNA viruses infection. Here, we demonstrate that the...

2023年4月14日 · Our RIP assay and in vitro binding assay revealed that UBAP2L association with G3BP1 was mediated by snoRNAs. There are two types of snoRNAs, C/D box snoRNAs and H/ACA box snoRNAs.

2023年6月22日 · In this study, we developed targets of RBPs identified by editing induced through dimerization (TRIBE-ID), a facile strategy for quantifying state-specific RNA–protein interactions upon...

The stress granule protein G3BP1, known to arrest mRNA translation, was identified as a regulator of RIG-I–induced IFN-β production. How G3BP1 functions in RIG-I signaling is not known, however.

2025年2月6日 · Ribonucleoprotein (RNP) granules have been linked to translation regulation and disease, but their assembly and regulatory mechanisms are not well understood. Here, we show that the RNA-binding protein G3BP1 preferentially interacts with unfolded RNA, driving the assembly of RNP granule-like condensates that establish RNA-RNA interactions.

2020年5月14日 · To study transcript partitioning under cell stress, we catalogued mRNAs enriched in prostate carcinoma PC-3 cell PSs, as defined by polysome fractionation and RNA sequencing (RNAseq), and compared them to mRNAs complexed with the known SG-nucleator protein, G3BP1, as defined by spatially-restricted enzymatic tagging and RNAseq.

2022年5月5日 · Ras-GTPase-activating protein binding protein 1 (G3BP1) is a multifunctional binding protein involved in a variety of biological functions, including cell proliferation, metastasis, apoptosis, differentiation and RNA metabolism.

2023年12月12日 · To investigate the mechanism by which G3BP1 activates PI3K/Akt signaling, we performed RNA immunoprecipitation sequencing (RIP-seq) using UMUC3 cells that stably express HA-G3BP1. Notably, we revealed that G3BP1 binds to the mRNAs of class IA PI3Ks (PIK3CA, PIK3CB, PIK3CD, and PIK3R1) through multiple motifs ( …

2024年11月6日 · Ras-GTP 酶激活蛋白结合蛋白 1 （G3BP1） 是应激颗粒 （SGs） 的核心组分和关键调节开关。 当细胞内游离 RNA 的浓度增加时，它可以触发以 G3BP1 为核心的 RNA 依赖性液-液相分离 （LLPS），从而形成影响细胞存活或死亡的 SG。 此外，G3BP1 与各种宿主蛋白相互作用以调节 SGs 的表达。 作为一种多功能结合蛋白，G3BP1 具有多种生物学功能，影响细胞增殖、分化、凋亡和 RNA 代谢，是 Rac1-PAK1、TSC-mTORC1、NF-κB 和 STAT3 等信号通路 …