2024年12月25日 · RNF168 (Ring Finger Protein 168) is a Protein Coding gene. Diseases associated with RNF168 include Riddle Syndrome and Digeorge Syndrome. Among its related pathways are HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) and DNA Double Strand Break Response.

ATM激活后可以将MDC1（mediator of DNA damage checkpoint protein 1）磷酸化，再募集E3泛素连接酶RNF168，后者将组蛋白H2A泛素化，从而与53BP1（p53结合蛋白1）结合。 53BP1可以促进NHEJ并抑制HR。

通过IR-免疫荧光、免疫组化染色、CSR（class-swith recombination）等实验，进一步验证了生理条件下，RNF168-S60磷酸化（RNF168-SE突变模拟磷酸化）后，小鼠的DNA损伤修复功能受限，出现诸如LPS刺激后免疫球蛋白类别转换重组能力减弱，小肠绒毛在辐照损伤后再生变慢等 ...

2025年2月19日 · RNF168 suppresses the cancer stem cell-like traits of nonsmall cell lung cancer cells by mediating RhoC ubiquitination. RNF168-mediated localization of BARD1 recruits the BRCA1-PALB2 complex to DNA damage. RNF168 promotes RHOC degradation by ubiquitination to restrain gastric cancer progression via decreasing HDAC1 expression.

2024年10月11日 · Here we developed chemical strategies and determined the cryo-electron microscopy structures of the RNF168–UbcH5c~Ub–nucleosome complex captured in transient H2A K13/15 monoubiquitination and...

2024年1月18日 · RNF168 controls the chromatin recruitment of several DNA damage response (DDR) proteins, but its mechanisms of action remain unclear. Hu et al. unveil how RNF168 selectively ubiquitylates histone H2A in the nucleosome and reads its own ubiquitylation products, allowing for propagation on chromatin of ubiquitin marks recognized by DDR proteins.

2009年6月5日 · We identified and characterized RNF168, a new chromatin-associated RING finger protein. We demonstrated that RNF168 is endowed with ubiquitin ligase activity both in vitro and in vivo, which targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains.

环指蛋白 168 (rnf168) 是一种具有环指结构域的 e3 泛素连接酶。 它是一种重要的蛋白质，有助于 DNA 双链损伤修复途径。 最近的研究发现RNF168与多种癌症的发生发展有显着关系。

该基因编码包含无名指的 E3 泛素连接酶蛋白，无名指是存在于多种功能不同的蛋白质中的基序，已知参与蛋白质-DNA 和蛋白质-蛋白质相互作用。 该蛋白质参与 DNA 双链断裂 (DSB) 修复。 该基因的突变会导致 Riddle 综合征。 [RefSeq 提供，2011 年 9 月]

RNF168 plays a central role in the DNA damage response (DDR) by ubiquitylating histone H2A at K13 and K15. These modifications direct BRCA1-BARD1 and 53BP1 foci formation in chromatin, essential for cell-cycle-dependent DNA double-strand break (DSB) repair pathway selection.