2020年6月9日 · To examine the individual impacts of ROPs and GRAs on host gene expression, we developed a robust, novel protocol to enrich for ultrapure populations of a naturally occurring and reproducible population of host cells called uninfected-injected (U-I) cells, which Toxoplasma injects with ROPs but subsequently fails to invade.

2024年1月3日 · GRA effectors are also found at the PVM: GRA15 controls NF-κB gene expression 106; GRA7 works with ROP18 to neutralize IRGs 48; and GRA6 controls NFAT4 to increase CCL2 and CXCL2 expression 8 ...

genes but also various unreported non-ROP/GRA genes are identified as IFN-g-dependent or -independent in vivo fitness genes by comparison of screen results using immune-compe-tent wild-type (WT) and immune-deficient (Ifngr1 /) mice. The current study provides the community with a genetic resource

2019年12月1日 · Toxoplasma introduces ROP and GRA effectors into host cells to coopt host functions. ROP effector secretion occurs during invasion via an unknown mechanism. MYR1-3, ROP17, and ASP5 are required for transit of GRAs across Toxoplasma ’s PVM.

2019年9月6日 · Toxoplasma gondii secretes rhoptry (ROP) and dense granule (GRA) effector proteins to evade host immune clearance mediated by interferon gamma (IFN-γ), immunity-related GTPase (IRG) effectors, and CD8 + T cells. Here, we investigated the role of parasite-secreted effectors in regula …

2019年9月6日 · Toxoplasma gondii secretes rhoptry (ROP) and dense granule (GRA) effector proteins to evade host immune clearance mediated by interferon gamma (IFN-γ), immunity-related GTPase (IRG) effectors, and CD8 + T cells.

2020年2月4日 · To examine the individual impacts of ROPs and GRAs on host gene expression, we developed a robust, novel protocol to enrich for ultra-pure populations of a naturally occurring and reproducible population of host cells called uninfected-injected (U-I) cells, which Toxoplasmainjects with ROPs but subsequently fails to invade.

2020年4月14日 · These ROP-dependent pro-inflammatory signatures appear to be counteracted by at least some of the MYR1-dependent GRAs and may be enhanced by the MYR-independent GRAs, (which are found embedded within the PVM).

2020年6月9日 · To examine the individual impacts of ROPs and GRAs on host gene expression, we developed a robust, novel protocol to enrich for ultrapure populations of a naturally occurring and reproducible...

2019年10月1日 · Recently, many studies started to focus on T. gondii antigens belonging to surface antigens (SAGs), and excretory - secretory antigens (ESA) such as micronemal proteins, rhoptry proteins (ROPs) and dense granule proteins (GRAs) that have been shown to play a major role in stimulating protective immunity (Dlugonska, 2008; Jongert et al., 2008; We...