2023年2月1日 · In this study, we found for the first time that TMZ can produce reactive oxygen species (ROS) under the influence of ultrasound (US). This property allows TMZ-US therapy to have better efficacy in the treatment of GBM.

2024年3月4日 · In TMZ-resistant cells, the depletion of ITPKB led to an increase in reactive oxygen species (ROS) related to NADPH oxidase (NOX) activity and restored cell sensitivity to TMZ....

2019年10月24日 · Starting from ROS analysis, U251 TMZ-responsive cells are characterized by a lower basal level of cytoplasmic ROS compared to T98 TMZ-resistant ones, and only in sensitive cells, TMZ can induce an increase of cytoplasmic ROS. TMZ-induced ROS are fundamental for GBM cell responsiveness driving CMA activation, the expression of pro-apoptotic ...

近日， 中南大学湘雅医院 研究团队 在国际权威学术期刊 Signal Transduction And Targeted Therapy （IF=39.3）上发表了题为“ Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis ”的最新研究成果。 本研究对配对的原发性和复发性GBM患者组织样本进行 蛋白质组学 分析， 确定了 肌醇1,4, 5-三磷酸 （IP3）激酶B（ITPKB）可以作为克服TMZ耐药的新靶点，揭示了 E3泛素连接酶 Trim25介导的ITPKB泛 …

2019年10月24日 · Sensitive and resistant GBM cells were used to test the role of mitochondrial ROS release in TMZ-resistance. Chaperone-Mediated Autophagy (CMA) activation in relation to reactive oxygen species (ROS) release has been measured by monitoring the expression of …

CN-3, a new asterosaponin, showed cytotoxic effects on TMZ-resistant cells in a dose- and time-dependent manner via reactive oxygen species (ROS)-mediated apoptosis and autophagy. Transmission electron microscopy and monodansylcadaverine (MDC) staining showed turgidity of the mitochondria and autophagosomes in CN-3-treated SHG44R and U87R cells.

该研究首次发现TMZ在超声 (US)的影响下会产生活性氧 (ROS)。 这一特性使得TMZ-US疗法在GBM治疗中具有更好的疗效。 鉴于US在中枢神经系统 (CNS)疾病中的使用日益增多，以及TMZ对GBM治疗的重要性，有助于TMZ对相关胶质母细胞瘤治疗的发展。 中科新生命 为该研究提供了4D Labelfree蛋白质组学技术服务。 步骤5：TMZ-US不能诱导线粒体依赖性细胞凋亡。 1. TMZ-US在体外增强TMZ对胶质瘤的化疗敏感性. 在本研究中，作者采用不同浓度的TMZ联合相同超声处 …

2024年3月4日 · In TMZ-resistant cells, the depletion of ITPKB led to an increase in reactive oxygen species (ROS) related to NADPH oxidase (NOX) activity and restored cell sensitivity to TMZ. Mechanistically, the decreased phosphorylation of the E3 ligase Trim25 at the S100 position in recurrent GBM samples accounted for the weakened ITPKB ubiquitination.

TMZ induced reactive oxygen species (ROS) burst and elevated G2 arrest in glioma cells. The CINN-suppressed ROS burst in TMZ-treated glioma cells might be associated with increased apoptosis, as indicated by the upregulation of TUNEL-positive glioma cells.

提高高 ROS 水平的缺氧条件加速了原位TMZ 从抗 PD-L1-TKNPs 中释放。 TMZ 产生的超积累 ROS 引起氧化损伤，导致线粒体介导的细胞凋亡。 在多功能纳米系统（抗PD-L1-TMZ-TKNPs）中制造的TMZ在体内条件下提供了极好的肿瘤积累和延缓肿瘤生长。 在用抗 PD-L1-TMZ-TKNPs 治疗后，随着凋亡标记物、DNA 双链断裂标记物的激活和肿瘤组织中血管生成标记物的下调，细胞凋亡效应的增强发挥了强大的抗癌作用。 总的来说，纳米结构提供了深入的肿瘤渗透和高药物 …