2021年4月13日 · Here, we report efficiency of cytosine base editors (CBEs) in Arabidopsis thaliana by applying the strong endogenous RPS5a promoter to drive the expression of …

Gateway destination vector for plants to express ORFs from entry clone with RecA1 plastid targeting signal under RPS5A promoter.

2021年12月31日 · The RPS5A promoter-driven SpCas9 successfully produced monoallelic mutations at three target genes in N. attenuata seeds with TRV-delivered guide RNA. These …

Plasmid pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus from Dr. Dolf Weijers's lab contains the inserts mDII, ntdTomato, DII, and n3xVenus and is published in Nat Methods. …

Here, we present a newly developed, highly efficient CRISPR/Cas9 vector for Arabidopsis thaliana, pKAMA-ITACHI Red (pKIR), harboring the RIBOSOMAL PROTEIN S5 A (RPS5A) …

作者开发了一种基因编码的、完全可逆的生物传感器，用于从细菌色氨酸抑制因子 (Trpr)开始，以高空间和时间分辨率对生长素梯度进行体内成像。 图 1. IAA类似于色氨酸：都含有吲哚环， …

与病毒来源的强异源CaMV35S启动子相比，RPS5a启动子将CBE效率提高了32%，当靶向LFY基因时，T 1植物的数量显示出超过50%的转化率。 CBE 通过 C-to-T 转换诱导LFY发生无义突 …

2021年4月13日 · Here, we report efficiency of cytosine base editors (CBEs) in Arabidopsis thaliana by applying the strong endogenous RPS5a promoter to drive the expression of …

The RPS5a promoter has been reported to be among the most efficient in driving Cas9 expression in A. thaliana [28, 36], a dicotyledonous plant species such as V. vinifera. These …

Using an optimized genome editing system with RPS5a promoter-driven Cas9, putatively quintuple mutant lines lacking detectable amounts of Lhcb1 protein represented approximately …