These CST™ antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.In this assay, PTMScan ® (RXXS

Chart showing the proportions of underlying Akt substrate-like sequence motifs found in an Akt substrate PTMScan ® study using Akt Substrate Motif mAb 1. Analysis of three cell lines gave 281 non-redundant peptide sequences containing Akt substrate-like motifs (Moritz et al., Akt-RSK-S6 kinase signaling networks activated by oncogenic receptor ...

In this assay, PTMScan ® (RXXS*/T*) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the RXXS*/T* motif (S* = phospho-serine, T* = phospho-threonine).

In this assay, PTMScan® (RXXS*/T*) Motif Antibody bead conjugates are used to specifically enrich phospho-pep-tides containing the RXXS*/T* motif (S*= phospho-serine, T*= phospho-threonine). RXRXXS* 31% RXXT* 21% RXRXXT* 5% RXXS* 43% Chart showing the proportions of underlying Akt substrate-like sequence motifs found in an Akt substrate ...

Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug ...

RXRXXS/T motif were identified with PTMScan® Technology using Phospho-Akt substrate antibodies. These Cell Signaling Technology antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

AMPK Substrate Motif (LXRXXS */T*) Rabbit mAb contained in this kit, which binds to the AMPK phosphorylation motif LXRXXS*/T* (4), was developed by CST for use in the study and discovery of new AMPK substrates. In this assay, PTMScan® (LXRXXS*/T*) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides

2 天之前 · Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug ...

2013年5月10日 · The RxxS is a known motif identified in SnRK2s substrate proteins (21, 22). The absence of this motif in the phosphopeptide datasets from the snrk2.2/2.3/2.6 triple mutant further supports its function as a phosphor motif specific for the SnRK2s.

2011年1月1日 · The phosphorylation domains C1, C2, and C3 contain two different recognition motifs (motif I and motif II) for Ser/Thr protein kinases. Motif I (RXXS/T) is a target sequence for calcium-regulated protein kinases, such as CDPK and calcium/calmodulin dependent protein kinase II (CaMKII), and is recognized by SnRK2 family kinases ( Chae et al ...