Novel Diubiquitin Probes Expand the Chemical Toolkit to …
2016年4月21日 · To be able to study DUBs that use S2 pocket binding to modulate DUB activity and specificity, Flierman et al. (2016) designed a set of novel probes based on non …
A deubiquitylase with an unusually high-affinity ubiquitin-binding ...
2020年5月11日 · The co-crystal structure of the DUB (OtDUB) domain with ubiquitin revealed three bound ubiquitins: one engages the S1 site, the second binds an S2 site contributing to …
Discovery and mechanism of K63-linkage-directed deubiquitinase …
2024年11月25日 · Here, we report the discovery that USP53 and USP54 are active DUBs with high specificity for K63-linked polyubiquitin. We demonstrate how USP53 mutations abrogate …
Non-hydrolyzable Diubiquitin Probes Reveal Linkage-Specific …
2016年4月21日 · Although Ub (chain)-based active site-directed probes and activity reagents have proven excellent tools to both identify and characterize DUB activity and specificity for S1 …
Activity-Based Ubiquitin Probes for Investigation of Deubiquitinases
Polyubiquitin probes to address multiple auxiliary ubiquitin binding sites (beyond S2/S1 or S1/S1′binding modes) are highly desirable in investigating how DUBs process linear, mixed …
Catching a DUB in the act: novel ubiquitin-based active site …
2014年12月1日 · The proposed S1 and S1′ sites position a polyubiquitin chain across the active-site to allow cleavage between ubiquitin molecules in a chain. The S1′ site binds the ubiquitin …
OTU Deubiquitinases Reveal Mechanisms of Linkage ... - Cell Press
2013年7月3日 · Biochemical analysis and five crystal structures of OTU DUBs with or without Ub substrates reveal four mechanisms of linkage specificity. Additional Ub-binding domains, the …
Deubiquitinases: From mechanisms to their inhibition by small molecules
2022年1月6日 · Deubiquitinases (DUBs) are specialized proteases that remove ubiquitin from substrates or cleave within ubiquitin chains to regulate ubiquitylation and therefore play …
Novel Diubiquitin Probes Expand the Chemical Toolkit to Study …
2016年4月4日 · To be able to study DUBs that use S2 pocket binding to modulate DUB activity and specificity, Flierman et al. (2016) designed a set of novel probes based on non …
Chemical tools for structural studies of ubiquitin and ubiquitin …
2023年1月1日 · Crystal structures of DUBs complexed with mono-ubiquitin or UbL ABPs have been invaluable tools for understanding the recognition mechanism of the distal ubiquitin or …