Here, employing fluorescent probes and a tool for triggering ectodomain shedding, we show that epiregulin (EREG), a low-affinity EGFRL, rapidly and efficiently activates EGFR in Madin-Darby canine kidney (MDCK) epithelial cells and mouse epidermis.

2014年2月13日 · In this study, we describe the unexpected deposition of Erk2 on developmental promoters, as well as the corresponding decrease in RNAPII(S5P) upon Erk1/2 inactivation. Notably, the reduction in RNAPII(S5P) is specific to developmental genes and not active ones.

Epidermal growth factor receptor (EGFR) signaling pathways leading to G1/S cell cycle progression activated by EGF activation. Depicted are the RAS-RAF-MEK-ERK MAPK and PI3K-AKT-mTOR pathways. EGF activation of the EGFR induces receptor dimerization and transphosphorylation of the C-terminal domain.

2024年6月15日 · Here, we show that an EGFR endocytic mechanism, non-clathrin endocytosis (NCE), which is activated only at high ligand concentrations and targets receptor to degradation, requires a tripartite...

2025年2月19日 · EGF binds EGFR, and promotes p-ERK activation, which increases the expression of mesenchymal marker ZEB1 and decreases the expression of the epithelial marker miR-205, favouring mesenchymal phenotypic changes. Upon activation, EGFR undergoes ubiquitination and degradation, reducing EGFR expression on plasma membrane.

2020年8月11日 · Here, we developed a model explicitly accounting for conformational changes of the kinase and extracellular domains, their dimerizations and ligand binding to monomeric and dimeric receptor species. The model was fitted to ligand binding data of suspended cells expressing receptors with active or inactive kinase conformations.

2008年6月1日 · EGFR activation underlies the hyperproliferative effects of retinoids on skin keratinocytes. Indeed HB-EGF is markedly induced in keratinocytes in response to retinoid treatment, both in vitro and in vivo, and retinoid-dependent hyperproliferation is impaired by EGFR inhibition (Stoll and Elder, 1998; Varani et al., 2001).

2003年3月10日 · The epidermal growth factor (EGF) receptor (EGFR) is one of four homologous transmembrane proteins that mediate the actions of a family of growth factors including EGF, transforming growth factor-α, and the neuregulins.

2023年12月3日 · The binding of soluble EGF ligands to the extracellular domain promotes receptor activation. The epidermal growth factor receptor (EGFR) family is a subclass of receptor tyrosine kinase (RTK) proteins and consists of 4 members: EGFR (ErbB1, HER1), ErbB2 (HER2, neu in rodents), ErbB3 (HER3) and ErbB4 (HER4).

2018年10月1日 · Research on the epidermal growth factor (EGF) family and the family of receptors (EGFR) has progressed rapidly in recent times. New crystal structures of the ectodomains with different ligands, the activation of the kinase domain through oligomerisation and the use of fluorescence techniques have re …