Rapid single-step separation of pluripotent mouse embryonic …
The primary objective of the current investigation was to develop and validate a rapid, single-step separation technique for the complete removal of MEF feeder cells from mES cells. A …
addition of Wnt1 CM to both MEF-1 and MEF-7 signifi cantly increased the number of undifferentiated colonies, while the addition of Sfrps promoted differentiation. Together, these …
Efficient Derivation and Genetic Modifications of Human …
2012年8月8日 · Conventional procedures of these processes require co-culture with primary mouse embryonic fibroblasts (MEFs) to support self-renewal and clonal growth of human …
A novel method for generating xeno-free human feeder cells for …
Long-term cultures of human embryonic stem (hES) cells require a feeder layer for maintaining cells in an undifferentiated state and increasing karyotype stability. In routine hES cell culture, …
图像融合网络的通用评估指标 - CSDN博客
2024年3月14日 · 差异相关性总和( sum of correlation differ-ences, SCD) 测量融合图像与源图像的差异来表征融合算法优劣，D X,F 表示融合图像 F 与源图像 X 的差分图像。 SCD 越高,意味着 …
Feeder-free maintenance of hESCs in mesenchymal stem cell
2009年3月24日 · A paracrine regulation was recently proposed in human embryonic stem cells (hESCs) grown in mouse embryonic fibroblast (MEF)-conditioned media (MEF-CM), where …
Simplification of culture conditions and feeder-free expansion …
2021年5月26日 · We report here simplified bESC culture conditions based on replacing custom base medium with a commercially available alternative and eliminating the need for MEF …
MEF2信号与人类疾病 - 知乎 - 知乎专栏
MEF2家族蛋白包括MEF2A、B、C、D，共享一个类似的N-末端，含有一个高度保守的MADS-box结构域和一个紧邻MEF2域。 MADS-box和MEF2域分别有55个和29个氨基酸，有助 …
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小鼠胚胎成纤维细胞（MEF
mef细胞分选自小鼠的胚胎，低温存 储之前，细胞经过存活率测试（冷冻复苏后的存活率>80%），并通过无菌、支原体检测。 MeilunCell ® 小鼠胚胎成纤维细胞取自怀孕13.5d 的KM …
2024年最新版SCD《科学引文数据库》来源期刊全目录！（附可检 …
2024年1月18日 · 《科学引文数据库》 (Science Citation Database 简称：SCD)，是我国第1个涵盖自然科学、工程与技术、农林科学、医药科学、人文学科、社会科学等全部非保密学科的大 …