The SCF ubiquitin ligase: insights into a molecular machine
2004年9月1日 · The SCF complex is a multi-subunit ubiquitin ligase that specifically transfers activated ubiquitin to target-protein substrates. SCF is named after Skp1–Cullin–F-box-protein, …
Ubiquitin ligation to F-box protein targets by SCF–RBR E3
2021年2月3日 · Neddylated SCF-activated ARIH1 binds thioester-bonded UBE2L3~ubiquitin (Ub), mimicked by ubiquitin isopeptide bonded to a mutant UBE2L3 in which the catalytic cysteine is …
Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase …
2002年4月18日 · SCF complexes are the largest family of E3 ubiquitin–protein ligases and mediate the ubiquitination of diverse regulatory and signalling proteins. Here we present the …
Priming and Extending: A UbcH5/Cdc34 E2 Handoff Mechanism …
2010年3月26日 · We describe a mechanistic model of polyubiquitination by the SCFβTrCP2 E3 ubiquitin (Ub) ligase using human IκBα as a substrate. Biochemical reconstitution experiments …
Identification of SCF Ubiquitin Ligase Substrates by Global Protein ...
2008年11月7日 · We established and applied genetic technologies that combine global protein stability (GPS) profiling and genetic perturbation of E3 activity to screen for substrates of the …
Dual BACH1 regulation by complementary SCF-type E3 ligases
2024年12月26日 · The ubiquitin (Ub)-proteasome system is a critical layer regulating NRF2 and BACH1 activity under physiological and oxidative stress conditions. While the inactivation of …
The SCF Ubiquitin Ligase: Molecular Cell - Cell Press
The SCF E3 ubiquitin ligases select specific proteins for ubiquitination (and typically destruction) by coupling variable adaptor (F box) proteins that bind protein substrates to a conserved …
Role of SKP1-CUL1-F-Box-Protein (SCF) E3 Ubiquitin
2013年3月20日 · Ubiquitin (Ub) is activated by the E1 ubiquitin-activating enzyme in an ATP-dependent reaction. Activated Ub is then transferred to an E2 ubiquitin-conjugating enzyme. …
Systemwide disassembly and assembly of SCF ubiquitin ligase …
2023年4月6日 · Cellular repertoires of SCF (SKP1-CUL1-F box protein) ubiquitin ligase complexes, which mediate much protein degradation, require CAND1 to distribute the limiting …
The SCF Complex Is Essential to Maintain Genome and …
The SKP1, CUL1, F-box protein (SCF) complex encompasses a group of 69 SCF E3 ubiquitin ligase complexes that primarily modify protein substrates with poly-ubiquitin chains to target …