2004年9月1日 · The SCF complex is a multi-subunit ubiquitin ligase that specifically transfers activated ubiquitin to target-protein substrates. SCF is named after Skp1–Cullin–F-box-protein, which are the...

Skp, Cullin, F-box containing complex (or SCF complex) is a multi-protein E3 ubiquitin ligase complex that catalyzes the ubiquitination of proteins destined for 26S proteasomal degradation. [1] Along with the anaphase-promoting complex, [2] SCF has important roles in the ubiquitination of proteins involved in the cell cycle.

2008年11月7日 · We established and applied genetic technologies that combine global protein stability (GPS) profiling and genetic perturbation of E3 activity to screen for substrates of the Skp1–cullin–F-box (SCF) ubiquitin ligase in mammalian cells.

2022年8月1日 · Here, we identified native components of the rice SCF D3 complex by a liquid chromatography–mass spectrometry (LC–MS) assay, purified them through chromatography, identified OsUBC14 as a specific E2 that works efficiently with the SCF D3 complex, and biochemically reconstituted activity of the SCF D3 E3 ligase in vitro.

Protein degradation orchestrated by SKP1·CUL1·F-box protein (SCF) ubiquitin ligases is a fundamental process essential for cellular and organismal function. The dynamic assembly of SCFs,...

2019年11月28日 · We review the biological roles of the SCF ubiquitin-ligase complex in gametogenesis, oocyte-to-embryo transition, embryo development and the regulation for estrogen and progestin.

SCF complexes are the largest family of E3 ubiquitin–protein ligases and mediate the ubiquitination of diverse regulatory and signalling proteins. Here we present the crystal structure of the...

2016年3月14日 · Herein we describe a strategy to inhibit SCF E3 ligases using engineered ubiquitin-based binders. We identify a previously uncharacterized inhibitory site and design ubiquitin-based libraries targeting this site.

2004年9月1日 · The F-box family of proteins - which are the substrate-recognition components of the Skp1-Cul1-F-box-protein (SCF) ubiquitin ligase - are important players in many mammalian functions. Here we explore a unifying and structurally detailed view of SCF-mediated proteolytic control of cellular processes that has been revealed by recent studies.

2004年3月11日 · Skp2 and its cofactor Cks1 are the substrate-targeting subunits of the SCF (Skp2-Cks1) (Skp1/Cul1/F-box protein) ubiquitin ligase complex that regulates entry into S phase by inducing the degradation of the cyclin-dependent kinase inhibitors p21 and p27 (ref. 1). Skp2 is an oncoprotein that often sho …