As an absolute technique for determining molar mass and size in solution, SEC-MALS - the combination of size-exclusion chromatography with multi-angle light scattering – offers an advanced characterization technique and overcomes the many limitations of column calibration.

Multi-Angle light scattering (MALS) describes a technique for measuring the light scattered by a sample into a plurality of angles. It is used for determining both the absolute molar mass and the average size of molecules in solution, by detecting how they scatter light.

Size exclusion chromatography with multi-angle static light scattering (SEC-MALS) is used to accurately measure weight-averaged masses (M w) of macromolecules in solution by measure the intensity of scattered light of a sample as it elutes from an SEC column.

Multi-Angle Light Scattering (MALS) is used to accurately measure the intensity of scattered light. Light scattering intensity is proportional to weight-averaged molar mass in solution. Combining SEC, MALS and concentration detectors in an SEC-MALS experiment allows for more accurate mass measurements that SEC or MALS alone.

2019年6月20日 · This protocol for SEC-MALS analyzes the molecular weight and size of pure protein monomers and aggregates. The data acquired serve as a foundation for further SEC-MALS analyses including those of complexes, glycoproteins and …

Absolute molar mass and size distributions of macromolecules in solution, combining a DAWN ® or miniDAWN ® multi-angle static light scattering (MALS) instrument with size exclusion chromatography (SEC/GPC).

A multi-angle light scattering (MALS) detector is a form of static light scattering detector that allows the absolute molecular weight (Mw) and potentially the radius of gyration (Rg) of a sample to be measured.

This tutorial review presents the theory and application of SEC-MALS with minimal equations and a focus on synthetic polymer characterization, serving as an entry point for polymer scientists who want to learn more about SEC-MALS.

2014年5月27日 · The most commonly known version is SEC-MALS (sometimes also referred to as SEC-MALLS for multiple angle laser light scattering). MALS measures the light scattered by the samples at a range of angles and then extrapolates back to zero degrees.

2025年3月13日 · Explore how SEC-MALS enhances protein analysis by providing accurate molecular weight and aggregation data for biopharmaceutical research and development. Size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) is a powerful technique for analyzing proteins in solution.