The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. Applications include use as a transfection host or in immunology, neuroscience, and toxicology research.

SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.

Transformed neuronal-like cell lines can be used in vitro to overcome this limitation. However, several caveats exist when utilizing cells derived from malignant tumors. In this context, the popular SH-SY5Y neuroblastoma cell line and its use in in vitro systems is described.

2021年9月8日 · The SH-SY5Y cell line is a comparatively homogeneous neuroblast-like cell line, which exhibits neuronal marker enzyme activity (tyrosine and dopamine-β-hydroxylases), specific uptake of norepinephrine (NA), and expresses one or more neurofilament proteins. SH SY5Y cells also express opioid, muscarinic, and nerve growth factor receptors.

SH-SY5Y is a human-derived cell line that originated from subcloning of the SK-N-SH neuroblastoma cell line in 1970. The parent cell line, SK-N-SH, was developed from the bone marrow biopsy of a four-year-old female neuroblastoma patient.

2022年9月1日 · The SH-SY5Y human neuroblastoma cell line (ATCC® CRL-2266™) is one of the most used cell lines in neurosciences, either undifferentiated or differentiated into neuron-like cells.

2025年2月11日 · Explore the unique features and growth phases of SH-SY5Y cells, focusing on their receptor expression and signal transduction pathways. SH-SY5Y cells, a human-derived neuroblastoma cell line, are widely used in scientific research due to their versatility and relevance to neurological studies.

In this study, we present an optimized neuronal differentiation protocol for SH-SY5Y that requires only two work steps and 6 days. After differentiation, the cells present increased levels of ATP and plasma membrane activity but reduced expression of energetic stress response genes.

2024年2月27日 · SH-SY5Y human neuroblastoma cells are one of the most widely used cellular models to study neurodegenerative diseases 4. SH-SY5Y cells can proliferate continuously and as undifferentiated...

Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.