2013年8月22日 · Conversely, the protein deacetylase sirtuin 2 (SIRT2) deacetylates and destabilizes ACLY. Substitution of 3K abolishes ACLY ubiquitylation and promotes de novo lipid synthesis, cell proliferation, and tumor growth. Importantly, 3K acetylation of ACLY is increased in human lung cancers.

2024年3月1日 · Currently, acetylation of ACLY at Lys-540, Lys-546 and Lys-554 makes ACLY more stable by antagonizing ACLY ubiquitination, and ACLY-3K acetylation can also promote the stability of ACLY protein in mouse liver cell line AML12, while SIRT2 promotes the deacetylation of ACLY-3K, resulting in the reduction of ACLY stability. 50 In the present study ...

在这项研究中，我们发现了 SIRT6 在控制 ACLY 和 ACLY 依赖性肿瘤抑制基因调控的核水平方面的新功能。 癌细胞中 SIRT6 的失活导致核 ACLY 蛋白的积累并增加核乙酰辅酶 A 库，进而驱动位点特异性组蛋白乙酰化以及促进肿瘤侵袭的癌细胞粘附和迁移基因的表达。 我们的研究结果揭示了 SIRT6 抑制侵袭性癌细胞表型的新机制，并将乙酰辅酶 A 响应性细胞迁移和粘附基因确定为 SIRT6 …

2024年3月1日 · sirt2抑制剂agk2可以抑制食道鳞状细胞癌的增殖和迁移，并增加acly蛋白质的乙酰化水平。 这些结果表明，SIRT2/ACLY信号轴的靶向治疗可能是食道鳞状细胞癌的潜在治疗策略。

2024年5月10日 · Ten DEGs associated with FAO were identified by RNA-Seq data, and ACLY, which generates acetyl-CoA from citrate for fatty acid and cholesterol biosynthesis, was found to be one of the most altered genes. ACLY was negatively mediated by SIRT1 in vivo and in vitro, as determined by western blot analysis.

Numerous studies have shown that lipid synthesis can be affected by regulating the acetylation state of ACLY through SIRT2 and P300/calcium-binding protein (CBP)-associated factor (PCAF) acetyltransferases. SIRT2 deacetylates ACLY for ubiquitination and degradation, inhibiting de novo lipid synthesis. P300/PCAF catalyses the opposite effect [41, 42

2024年11月15日 · Functional investigations demonstrated SIRT3’s regulation of benzoylated protein peptidyl-prolyl cis -trans isomerase F (PPIF), where K73bz and K197bz markedly diminished interactions with the tumor suppressor p53. Additionally, K978bz on ATP-citrate lyase (ACLY) notably inhibited its enzymatic activity.

The SIRT2 inhibitor AGK2 significantly increased the acetylation level of ACLY protein and inhibited the proliferation and migration of ESCC cells, while overexpression of ACLY partially reversed the inhibitory effect of AGK2 on ESCC cells.

2024年3月1日 · SIRT2抑制剂AGK2显着增加ACLY蛋白的乙酰化水平并抑制ESCC细胞的增殖和迁移，而ACLY的过表达部分逆转了AGK2对ESCC细胞的抑制作用。 总的来说，这些结果表明针对 SIRT2/ACLY 信号轴可能是 ESCC 患者的潜在治疗策略。 ATP citrate lyase (ACLY), as a key enzyme in lipid metabolism, plays an important role in energy metabolism and lipid biosynthesis of a variety of tumours.

ATP-citrate lyase (ACLY) is upregulated or activated in several types of cancer, and inhibition of ACLY arrests proliferation of cancer cells. Here we show that ACLY is acetylated at lysine residues 540, 546, and 554 (3K).