Effects of shRNA-Mediated SOX9 Inhibition on Cell ... - PubMed
Expression of SOX9 mRNA and protein was the lowest in effectene + shRNA-SOX9 + UTMD group. Moreover, transfection of shRNA-SOX9 with UTMD and effectene in combination could markedly inhibit the proliferation and induced cell apoptosis of Hep3B cells.
SOX9 depletion attenuates retinal ganglion cell ferroptosis …
2024年4月1日 · The specific short hairpin (sh)-RNA vectors targeting SOX9 (sh-SOX9–1 and sh-SOX9–2) to knock down SOX9 and its negative control (sh-NC) were obtained from GenePharma (Shanghai, China).
Profibrotic role of the SOX9–MMP10–ECM biosynthesis axis in the ...
2024年9月1日 · We investigated the role of SOX9 in TGF-β1-induced fibroblast activation and ECM deposition in rat tracheal fibroblast (RTF) cells. SOX9 overexpression adenovirus (Ad-SOX9) and siRNA were transfected into RTF cells. We found that SOX9 expression was up-regulated in RTF cells treated with TGF-β1.
A novel short hairpin RNA (shRNA) expression system promotes Sox9 …
2009年7月1日 · The resulting shRNA expression system displays robust, Sox9-dependent gene silencing. Dependence on Sox9 expression was confirmed by electrophoretic mobility shift assays. The ability of the system to regulate heterologously expressed Sox9 was demonstrated by Western blot, as a function of both Sox9 to shRNA ratio, as well as time from ...
论文解读｜Hongyang Wang/Liwei Dong教授团队总结SOX9
2024年10月31日 · 复旦大学的Hongyang Wang教授团队和海军军医大学的Liwei Dong教授团队在本刊发表了题为“Diverse functions of SOX9 in liver development and homeostasis and hepatobiliary diseases”的综述。该综述系统地总结了SOX9在肝胆发育、稳态和疾病中的最新研究进展，并强调了SOX9作为新型生物 ...
转录因子SOX9基因对脑胶质瘤干细胞干性维持的相关性研究-【维 …
摘要 目的:应用慢病毒干扰sox9的表达,观察其对脑胶质瘤干细胞干性维持的影响。方法:设计2条针对sox9转录短发夹rna(sh rna)的dna序列,构建慢病毒并感染胶质瘤细胞系u87、u251细胞,利用嘌呤霉素筛选稳转细胞。
LncRNA SOX9-AS1 triggers a transcriptional program involved in …
2024年1月17日 · SOX9-AS1 silencing significantly increases the migration and invasion of MDA-MB-468 cells (p value ≤ 0.05 and < 0.01 for sh-SOX9-AS1-1 and sh-SOX9-AS1-2, respectively) (Fig. 9C) and...
Transcription Factor Sox9 Exacerbates Kidney Injury through …
Our study investigated the possible mechanisms of the role of the transcription factor Sox9 in the development and progression of kidney injury through regulation of the miR-96-5p/Trib3/IL-6 axis. Bioinformatics analysis was performed to identify differentially expressed genes in kidney injury and normal tissues.
论文解读｜Hongyang Wang/Liwei Dong教授团队总结SOX9在肝发 …
复旦大学的Hongyang Wang教授团队和海军军医大学的Liwei Dong教授团队在本刊发表了题为“Diverse functions of SOX9 in liver development and homeostasis and hepatobiliary diseases”的综述。该综述系统地总结了SOX9在肝胆发育、稳态和疾病中的最新研究进展，并强调了SOX9作为 …
靶向SOX9调控弥漫性大B细胞淋巴瘤代谢重编程的研究
基于HPA数据库中的IHC研究，绝大多数原发性非霍奇金性淋巴瘤组织中检测不到SOX9的表达.在癌症细胞系百科全书（Cancer Cell Line Encyclopedia，CCLE）数据库中对各细胞系表达量的测定也显示，绝大多数DLBCL细胞株的SOX9表达量极低.此前的研究鉴定了低表达SOX9是ABC亚型的 ...